Institute of Pathology, University Hospital Bonn, D-53127 Bonn, Germany.
Int J Mol Med. 2011 Oct;28(4):605-11. doi: 10.3892/ijmm.2011.746. Epub 2011 Jul 8.
Prostate cancer (PCA) is a clinically heterogeneous and often multifocal disease with a clinical outcome difficult to predict. A deeper knowledge of the molecular basis of the disease may lead to a better prediction of prognosis. Therefore, in this study we investigated the molecular basis of PCA by identifying potential tumor markers in laser-microdisected PCA tissues. Among a group of PCA patients, quantitative RT-PCR analysis was performed to compare the expression of 70 genes. These genes were selected from the results of two microarrays which investigated the gene expression profile differences between moderately or poorly differentiated prostate carcinoma glands and the corresponding normal glands. Among the genes examined, CDKN2A, GATA3, CREBBP, ITGA2, NBL1 and TGM4 were down-regulated in the prostate carcinoma glands compared to the corresponding normal glands, whereas TFF3, TMPRSS2 and ERG were up-regulated. Our findings indicate that these genes may play roles as tumor suppressor genes or oncogenes in PCA, and may serve as potential tumor markers and novel therapeutic targets.
前列腺癌（PCA）是一种临床异质性的、常常呈多灶性的疾病，其临床结局难以预测。对疾病分子基础的更深入了解可能有助于更好地预测预后。因此，在本研究中，我们通过鉴定激光微切割 PCA 组织中的潜在肿瘤标志物来研究 PCA 的分子基础。在一组 PCA 患者中，进行了定量 RT-PCR 分析以比较 70 个基因的表达。这些基因是从两个微阵列的结果中选择的，该两个微阵列调查了中度或低度分化的前列腺癌腺与相应的正常腺之间的基因表达谱差异。在所检查的基因中，与相应的正常腺相比，CDKN2A、GATA3、CREBBP、ITGA2、NBL1 和 TGM4 在前列腺癌腺中下调，而 TFF3、TMPRSS2 和 ERG 上调。我们的研究结果表明，这些基因可能在 PCA 中作为肿瘤抑制基因或癌基因发挥作用，并且可能作为潜在的肿瘤标志物和新的治疗靶点。
