Fujise K, Nagamori S, Hasumura S, Homma S, Fujita K, Sujino H, Matsuura T, Shimizu K, Niiya M, Ohno T
First Department of Internal Medicine, Jikei University School of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1990 Sep;87(9):1851-5.
Authors have successfully established 7 human hepatocellular carcinoma (HCC) cell lines. In one of these cell lines, JHH-7, derived from HBs antigen positive HCC, hepatitis B virus (HBV) genome was analyzed by Southern blot hybridization. Digesting with Hind III restriction endonuclease, two bands were obtained at 6.0 and 2.5 kb, that is the integration of HBV genome was confirmed at the two sites of chromosomes in this cell line. And analyzing with 32P labeled HBV-DNA fragments for probes, it was indicated that integrated HBV genome was incomplete one containing from X to C region.
作者已成功建立了7种人肝细胞癌（HCC）细胞系。在其中一种细胞系JHH - 7中，该细胞系源自乙肝表面抗原阳性的肝癌，通过Southern印迹杂交分析了乙型肝炎病毒（HBV）基因组。用Hind III限制性内切酶消化后，在6.0和2.5 kb处获得两条带，这证实了该细胞系中HBV基因组在染色体的两个位点发生了整合。用32P标记的HBV - DNA片段作为探针进行分析，结果表明整合的HBV基因组是不完整的，包含从X区到C区。
