完整的LM细胞从细胞外ATP生成环磷酸腺苷。
Production of cyclic AMP from extracellular ATP by intact LM cells.
作者信息
Westcott K R, Engelhard V H, Storm D R
出版信息
Biochim Biophys Acta. 1979 Feb 19;583(1):47-54. doi: 10.1016/0304-4165(79)90308-8.
Intact LM cells, a line of cultured mouse fibroblasts, exhibited an adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) activity in the presence of exogenous [alpha-32P]ATP which was 20--30% of that observed with comparable preparations of lysed cells. The extent of NaF and prostaglandin E1 stimulation was comparable in intact cells and lysed cells. 96% of the added ATP and 92% of the cyclic AMP produced by intact cells could be isolated extracellularly in the incubation medium. Cellular integrity under assay conditions was monitored by trypan blue exclusion. These data suggest that LM cells contain an adenylate cyclase activity which is accessible to extracellular ATP.
完整的LM细胞系（一种培养的小鼠成纤维细胞），在存在外源性[α-32P]ATP的情况下，表现出腺苷酸环化酶（ATP焦磷酸裂解酶（环化），EC 4.6.1.1）活性，该活性是用可比的裂解细胞制剂所观察到活性的20%-30%。完整细胞和裂解细胞中氟化钠和前列腺素E1的刺激程度相当。完整细胞产生的96%的添加ATP和92%的环磷酸腺苷可以在孵育培养基中细胞外分离出来。通过台盼蓝排斥法监测测定条件下的细胞完整性。这些数据表明，LM细胞含有一种可被细胞外ATP作用的腺苷酸环化酶活性。
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