Edmonds B, Klein M, Dale N, Kandel E R
Department of Pharmacology, University College of London, United Kingdom.
Science. 1990 Nov 23;250(4984):1142-7. doi: 10.1126/science.2174573.
In Aplysia sensory and motor neurons in culture, the contributions of the major classes of calcium current can be selectively examined while transmitter release and its modulation are examined. A slowly inactivating, dihydropyridine-sensitive calcium current does not contribute either to normal synaptic transmission or to any of three different forms of plasticity: presynaptic inhibition, homosynaptic depression, and presynaptic facilitation. This current does contribute, however, to a fourth form of plasticity--modulation of transmitter release by tonic depolarization of the sensory neuron. By contrast, a second calcium current, which is rapidly inactivating and dihydropyridine-insensitive, contributes to release elicited by the transient depolarization of an action potential and to the other three forms of plasticity.
在培养的海兔感觉神经元和运动神经元中，可以在检测递质释放及其调节的同时，选择性地研究主要钙电流类型的作用。一种缓慢失活、对二氢吡啶敏感的钙电流，对正常突触传递以及三种不同形式的可塑性（突触前抑制、同突触抑制和突触前易化）均无作用。然而，这种电流对第四种可塑性形式——通过感觉神经元的强直去极化调节递质释放——有作用。相比之下，另一种钙电流迅速失活且对二氢吡啶不敏感，它对动作电位的瞬时去极化引发的递质释放以及其他三种可塑性形式有作用。
