Sundby C
Dept. of Biochemistry, University of Lund, Sweden.
FEBS Lett. 1990 Nov 12;274(1-2):77-81. doi: 10.1016/0014-5793(90)81333-j.
Isolated thylakoid membranes were found to be efficiently protected against photo-inhibition by sodium bicarbonate (20 mM NaHCO3) under both anaerobic and aerobic conditions. Furthermore, data are presented which indicate that the pronounced sensitivity to photo-inhibition under anaerobic compared to aerobic conditions is due to the removal of protecting bicarbonate, rather than oxygen, from the medium. A second type of bicarbonate effect on photo-inhibition, in apparent contradiction to the protective effect of added NaHCO3, is that thylakoid membranes that were depleted in their endogenous bicarbonate by treatment with formate were found to be less susceptible to photo-inhibition than thylakoids in the normal non-depleted state.
研究发现，在厌氧和好氧条件下，分离的类囊体膜均能被碳酸氢钠（20 mM NaHCO₃）有效保护免受光抑制。此外，所呈现的数据表明，与好氧条件相比，厌氧条件下对光抑制的明显敏感性是由于培养基中保护性碳酸氢盐而非氧气的去除。碳酸氢盐对光抑制的第二种作用，明显与添加 NaHCO₃ 的保护作用相矛盾，即经甲酸盐处理使其内源性碳酸氢盐耗尽的类囊体膜，比处于正常未耗尽状态的类囊体对光抑制更不敏感。
