一种改良和改进的精子酰胺酶活性检测方法。
A modified and improved assay for sperm amidase activity.
作者信息
Cross N L
机构信息
Department of Obstetrics and Gynecology, University of California, Davis 95616.
出版信息
J Androl. 1990 Sep-Oct;11(5):409-13.
An assay for sperm amidase activity has been modified so that many samples can be measured rapidly, making it more suitable for use in a clinical setting. The modified assay gave the same results (P greater than 0.05) as its lengthier parent assay (Kennedy et al, 1989). The measured enzyme activity was proportional to the number of sperm assayed over the range tested (3.5 x 10(5) to 2.1 x 10(6) sperm). The within-assay coefficient of variation was 7.8 +/- 1.8% (average +/- SE, n = 12), and the between-assay coefficient of variation was 7.2 +/- 1.2% (n = 3). The sensitivity was 7.6 microIU/well.
一种用于检测精子酰胺酶活性的测定方法已被改进，以便能够快速检测多个样本，使其更适合临床应用。改进后的测定方法与较长的原始测定方法（Kennedy等人，1989年）得出的结果相同（P大于0.05）。在所测试的范围内（3.5×10⁵至2.1×10⁶个精子），测得的酶活性与检测的精子数量成正比。批内变异系数为7.8±1.8%（平均值±标准误，n = 12），批间变异系数为7.2±1.2%（n = 3）。灵敏度为7.6微国际单位/孔。
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