Cory Giles
Peninsula College of Medicine and Dentistry, University of Exeter, Exeter, UK.
Methods Mol Biol. 2011;769:25-30. doi: 10.1007/978-1-61779-207-6_2.
The scratch-wound assay is a simple, reproducible assay commonly used to measure basic cell migration parameters such as speed, persistence, and polarity. Cells are grown to confluence and a thin "wound" introduced by scratching with a pipette tip. Cells at the wound edge polarise and migrate into the wound space. Advantages of this assay are that it does not require the use of specific chemoattractants or gradient chambers and it generates a strong directional migratory response, even in cell types that do not show robust responses in "single cell" migration assays. It is most reliably analysed when performed using time-lapse imaging, which can also yield valuable cell morphology/protein localisation information.
划痕实验是一种简单且可重复的实验，常用于测量基本的细胞迁移参数，如速度、持续性和极性。将细胞培养至汇合状态，然后用移液器吸头划痕制造一个薄的“伤口”。伤口边缘的细胞极化并迁移到伤口空间。该实验的优点是不需要使用特定的化学引诱剂或梯度室，并且即使在“单细胞”迁移实验中没有强烈反应的细胞类型中，也能产生强烈的定向迁移反应。使用延时成像进行实验时分析最为可靠，延时成像还能提供有价值的细胞形态/蛋白质定位信息。
