成像足体动力学与基质降解。
Imaging podosome dynamics and matrix degradation.
作者信息
Starnes Taylor W, Cortesio Christa L, Huttenlocher Anna
机构信息
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
出版信息
Methods Mol Biol. 2011;769:111-36. doi: 10.1007/978-1-61779-207-6_9.
Abstract
Invasive cell migration is critical for leukocyte trafficking into tissues. Podosomes are matrix-degrading adhesive structures that are formed by macrophages and are necessary for macrophage migration and invasion. Here, we describe methods for imaging and quantifying podosomes in primary human macrophages and in THP-1 cells, a monocyte cell line that can be differentiated to a macrophage-like state. Moreover, we outline detailed methods for live imaging of podosomes, which are highly dynamic, and for the quantification of rates of podosome turnover. Finally, we discuss methods for the quantitative analysis of matrix degradation on fluorescent-gelatin-coated cover slips.
摘要
侵袭性细胞迁移对于白细胞进入组织的过程至关重要。足体是由巨噬细胞形成的降解基质的粘附结构，是巨噬细胞迁移和侵袭所必需的。在这里，我们描述了在原代人巨噬细胞和THP-1细胞（一种可分化为巨噬细胞样状态的单核细胞系）中对足体进行成像和定量的方法。此外，我们概述了对高度动态的足体进行实时成像以及定量足体周转速率的详细方法。最后，我们讨论了在荧光明胶包被的盖玻片上对基质降解进行定量分析的方法。
相似文献
1
Imaging podosome dynamics and matrix degradation.成像足体动力学与基质降解。
Methods Mol Biol. 2011;769:111-36. doi: 10.1007/978-1-61779-207-6_9.
2
Fascin actin bundling controls podosome turnover and disassembly while cortactin is involved in podosome assembly by its SH3 domain in THP-1 macrophages and dendritic cells.Fascin肌动蛋白成束调控足体周转和拆解，而在THP-1巨噬细胞和树突状细胞中，cortactin通过其SH3结构域参与足体组装。
Biochim Biophys Acta. 2015 May;1853(5):940-52. doi: 10.1016/j.bbamcr.2015.01.003. Epub 2015 Jan 16.
3
Podosome-type adhesions and focal adhesions, so alike yet so different.足体样黏附与黏着斑，如此相似却又如此不同。
Eur J Cell Biol. 2008 Sep;87(8-9):491-506. doi: 10.1016/j.ejcb.2008.02.012. Epub 2008 Apr 15.
4
Podosome rings generate forces that drive saltatory osteoclast migration.足突环产生驱动破骨细胞跳跃式迁移的力。
Mol Biol Cell. 2011 Sep;22(17):3120-6. doi: 10.1091/mbc.E11-01-0086. Epub 2011 Jul 7.
5
L-plastin nanobodies perturb matrix degradation, podosome formation, stability and lifetime in THP-1 macrophages.L- 肌动蛋白纳米体扰乱了 THP-1 巨噬细胞中基质的降解、足突的形成、稳定性和寿命。
PLoS One. 2013 Nov 13;8(11):e78108. doi: 10.1371/journal.pone.0078108. eCollection 2013.
6
The Rho-specific guanine nucleotide exchange factor Plekhg5 modulates cell polarity, adhesion, migration, and podosome organization in macrophages and osteoclasts.Rho特异性鸟嘌呤核苷酸交换因子Plekhg5调节巨噬细胞和破骨细胞中的细胞极性、黏附、迁移和小窝体组织。
Exp Cell Res. 2017 Oct 15;359(2):415-430. doi: 10.1016/j.yexcr.2017.08.025. Epub 2017 Aug 26.
7
Macrophage podosomes go 3D.巨噬细胞足突进入三维空间。
Eur J Cell Biol. 2011 Feb-Mar;90(2-3):224-36. doi: 10.1016/j.ejcb.2010.07.011.
8
Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages.肌动蛋白核点动力学的 WASp 磷酸化调节：在巨噬细胞中对基质降解和趋化作用的影响。
J Cell Sci. 2009 Nov 1;122(Pt 21):3873-82. doi: 10.1242/jcs.051755. Epub 2009 Oct 6.
9
Podosome reformation in macrophages: assays and analysis.巨噬细胞中足体的重塑：检测与分析
Methods Mol Biol. 2013;1046:97-121. doi: 10.1007/978-1-62703-538-5_6.
10
Lysosomal cathepsin B participates in the podosome-mediated extracellular matrix degradation and invasion via secreted lysosomes in v-Src fibroblasts.溶酶体组织蛋白酶B通过v-Src成纤维细胞中分泌的溶酶体参与足体介导的细胞外基质降解和侵袭。
Cancer Res. 2008 Nov 15;68(22):9147-56. doi: 10.1158/0008-5472.CAN-07-5127.
引用本文的文献
1
Characterization of a L136P mutation in Formin-like 2 (FMNL2) from a patient with chronic inflammatory bowel disease.鉴定一位慢性炎症性肠病患者的formin-like 2（FMNL2）基因中的 L136P 突变。
PLoS One. 2021 May 27;16(5):e0252428. doi: 10.1371/journal.pone.0252428. eCollection 2021.
2
Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs.定点定向 MT1-MMP 转运和表面插入调节发育中的 NMJs 处的 AChR 聚集和重塑。
Elife. 2020 Mar 24;9:e54379. doi: 10.7554/eLife.54379.
3
TIMP-2 secreted by monocyte-like cells is a potent suppressor of invadopodia formation in pancreatic cancer cells.单核细胞样细胞分泌的 TIMP-2 是胰腺癌细胞侵袭伪足形成的有效抑制剂。
BMC Cancer. 2019 Dec 13;19(1):1214. doi: 10.1186/s12885-019-6429-z.
4
Podosome formation impairs endothelial barrier function by sequestering zonula occludens proteins.足突形成通过隔离闭锁蛋白来损害内皮屏障功能。
J Cell Physiol. 2020 May;235(5):4655-4666. doi: 10.1002/jcp.29343. Epub 2019 Oct 21.
5
Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail.组织蛋白酶 B 上调并介导高表达 Snail 的结肠腺癌 HT29 细胞外基质降解。
Cells. 2019 Feb 27;8(3):203. doi: 10.3390/cells8030203.
6
Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis.肌球蛋白-IIA 重链 S1943 磷酸化调节肿瘤转移。
Exp Cell Res. 2018 Sep 15;370(2):273-282. doi: 10.1016/j.yexcr.2018.06.028. Epub 2018 Jun 25.
7
GPCR Signaling Mediates Tumor Metastasis via PI3Kβ.G蛋白偶联受体信号传导通过PI3Kβ介导肿瘤转移。
Cancer Res. 2016 May 15;76(10):2944-53. doi: 10.1158/0008-5472.CAN-15-1675. Epub 2016 Mar 24.
8
Microfluidic device for simultaneous analysis of neutrophil extracellular traps and production of reactive oxygen species.用于同时分析中性粒细胞胞外诱捕网和活性氧生成的微流控装置
Integr Biol (Camb). 2016 Feb;8(2):243-52. doi: 10.1039/c5ib00225g. Epub 2016 Jan 25.
9
The F-BAR protein PSTPIP1 controls extracellular matrix degradation and filopodia formation in macrophages.F-BAR 蛋白 PSTPIP1 调控巨噬细胞细胞外基质降解和丝状伪足形成。
Blood. 2014 Apr 24;123(17):2703-14. doi: 10.1182/blood-2013-07-516948. Epub 2014 Jan 13.
10
Integrin-specific control of focal adhesion kinase and RhoA regulates membrane protrusion and invasion.整合素特异性调控黏着斑激酶和 RhoA 调节细胞膜突起和侵袭。
PLoS One. 2013 Sep 9;8(9):e74659. doi: 10.1371/journal.pone.0074659. eCollection 2013.
本文引用的文献
1
Impaired podosome formation and invasive migration of macrophages from patients with a PSTPIP1 mutation and PAPA syndrome.携带PSTPIP1突变和PAPA综合征患者的巨噬细胞的足体形成受损及侵袭性迁移受损。
Arthritis Rheum. 2010 Aug;62(8):2556-8. doi: 10.1002/art.27521.
2
Three-dimensional migration of macrophages requires Hck for podosome organization and extracellular matrix proteolysis.巨噬细胞的三维迁移需要 Hck 来组织足突和细胞外基质的蛋白水解。
Blood. 2010 Feb 18;115(7):1444-52. doi: 10.1182/blood-2009-04-218735. Epub 2009 Nov 6.
3
Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages.肌动蛋白核点动力学的 WASp 磷酸化调节：在巨噬细胞中对基质降解和趋化作用的影响。
J Cell Sci. 2009 Nov 1;122(Pt 21):3873-82. doi: 10.1242/jcs.051755. Epub 2009 Oct 6.
4
Methods in molecular biology. Macrophages and dendritic cells. Methods and protocols. Preface.分子生物学方法。巨噬细胞与树突状细胞。方法与方案。前言。
Methods Mol Biol. 2009;531:v-vi. doi: 10.1007/978-1-59745-396-7.
5
The expression of exogenous genes in macrophages: obstacles and opportunities.巨噬细胞中外源基因的表达：障碍与机遇
Methods Mol Biol. 2009;531:123-43. doi: 10.1007/978-1-59745-396-7_9.
6
Efficient non-viral transfection of THP-1 cells.THP-1细胞的高效非病毒转染
J Immunol Methods. 2009 May 31;344(2):109-15. doi: 10.1016/j.jim.2009.03.014. Epub 2009 Apr 5.
7
FBP17 Mediates a Common Molecular Step in the Formation of Podosomes and Phagocytic Cups in Macrophages.FBP17介导巨噬细胞中足体和吞噬杯形成的共同分子步骤。
J Biol Chem. 2009 Mar 27;284(13):8548-56. doi: 10.1074/jbc.M805638200. Epub 2009 Jan 20.
8
Regulation of podosome formation in macrophages by a splice variant of the sodium channel SCN8A.钠通道SCN8A的剪接变体对巨噬细胞中足体形成的调节作用。
J Biol Chem. 2009 Mar 20;284(12):8114-26. doi: 10.1074/jbc.M801892200. Epub 2009 Jan 9.
9
Lysosomal cathepsin B participates in the podosome-mediated extracellular matrix degradation and invasion via secreted lysosomes in v-Src fibroblasts.溶酶体组织蛋白酶B通过v-Src成纤维细胞中分泌的溶酶体参与足体介导的细胞外基质降解和侵袭。
Cancer Res. 2008 Nov 15;68(22):9147-56. doi: 10.1158/0008-5472.CAN-07-5127.
10
WASP and WIP regulate podosomes in migrating leukocytes.WASP和WIP调节迁移白细胞中的足体。
J Microsc. 2008 Sep;231(3):494-505. doi: 10.1111/j.1365-2818.2008.02062.x.
Zotero 插件