Sixt Michael, Lämmermann Tim
Institute of Science and Technology Austria, Am Campus 1, Klosterneuburg, Austria.
Methods Mol Biol. 2011;769:149-65. doi: 10.1007/978-1-61779-207-6_11.
Cell migration on two-dimensional (2D) substrates follows entirely different rules than cell migration in three-dimensional (3D) environments. This is especially relevant for leukocytes that are able to migrate in the absence of adhesion receptors within the confined geometry of artificial 3D extracellular matrix scaffolds and within the interstitial space in vivo. Here, we describe in detail a simple and economical protocol to visualize dendritic cell migration in 3D collagen scaffolds along chemotactic gradients. This method can be adapted to other cell types and may serve as a physiologically relevant paradigm for the directed locomotion of most amoeboid cells.
细胞在二维（2D）基质上的迁移遵循与在三维（3D）环境中完全不同的规则。这对于白细胞尤为重要，因为白细胞能够在没有粘附受体的情况下，在人工三维细胞外基质支架的受限几何结构内以及体内的间质空间中迁移。在这里，我们详细描述了一种简单且经济的方案，用于可视化树突状细胞在三维胶原蛋白支架中沿趋化梯度的迁移。该方法可适用于其他细胞类型，并可作为大多数阿米巴样细胞定向运动的生理相关范例。
