Carisey Alex, Stroud Matthew, Tsang Ricky, Ballestrem Christoph
Faculty of Life Sciences, The University of Manchester, Manchester, UK.
Methods Mol Biol. 2011;769:387-402. doi: 10.1007/978-1-61779-207-6_26.
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
本章介绍基于显微镜的光漂白后荧光恢复（FRAP）技术的应用。为了量化亚细胞区室中蛋白质的动力学，我们首先概述FRAP实验的一般要点，然后提供一份详细方案，说明如何测量和分析FRAP实验的最重要参数，如移动部分和恢复半衰期。
