Service d'Anatomie Pathologique, CHU, Université de Poitiers, Poitiers, France.
Prostate. 2012 Apr;72(5):542-8. doi: 10.1002/pros.21456. Epub 2011 Jul 11.
In order to better understand the biological significance of perineural invasion (PNI) in prostate cancer, we aimed to analyze in situ the expression of molecules involved in tumor growth or nerve trophicity.
Tissues from 66 radical prostatectomies performed for prostate cancer (40 with PNI and 26 without PNI) were selected and included in a tissue microarray (TMA): PNI areas (when available), cancer far from nerves, and nerves far from cancer. The expression of the following molecules was analyzed using immunohistochemistry on TMA slides: macrophage migration inhibitory factor (MIF) and its receptor CD74, EGF receptor (EGFR), heregulin (HRG) and its receptor ErbB3, and the proliferation marker Ki67.
Cancer cells in the PNI areas showed increased proliferation, EGFR and CD74 expression, when compared to cells far from nerves (P = 0.009, 0.0005, and 0.02, respectively). Moreover, cell proliferation and CD74 staining were increased in cancers with PNI features compared to cancers without PNI (P = 0.001), even when adjusting for Gleason score, tumor size, and pathological stage.
These results suggest that cancer cells in the PNI areas could acquired a growth advantage that could be triggered by the growth factor receptors EGFR and CD74.
为了更好地理解前列腺癌中神经周围侵犯（PNI）的生物学意义，我们旨在分析参与肿瘤生长或神经营养的分子的原位表达。
从 66 例因前列腺癌而行根治性前列腺切除术的患者中选择组织，并包含在组织微阵列（TMA）中：PNI 区域（如有）、远离神经的肿瘤和远离肿瘤的神经。使用 TMA 载玻片上的免疫组织化学分析以下分子的表达：巨噬细胞移动抑制因子（MIF）及其受体 CD74、表皮生长因子受体（EGFR）、人表皮生长因子受体 3（ErbB3）配体 HRG 及其受体 ErbB3 和增殖标志物 Ki67。
与远离神经的细胞相比，PNI 区域的癌细胞显示出增殖增加、EGFR 和 CD74 表达增加（P=0.009、0.0005 和 0.02）。此外，与无 PNI 的癌症相比，具有 PNI 特征的癌症中的细胞增殖和 CD74 染色增加（P=0.001），即使在调整了 Gleason 评分、肿瘤大小和病理分期后也是如此。
这些结果表明，PNI 区域的癌细胞可能获得了生长优势，这种优势可能是由生长因子受体 EGFR 和 CD74 触发的。
