Virus Research and Development, Statens Serum Institut, Copenhagen, Denmark.
APMIS. 2011 Aug;119(8):487-97. doi: 10.1111/j.1600-0463.2011.02763.x. Epub 2011 May 14.
For a CD8 epitope-based vaccine to match different geographic locations, the targeted epitopes for cytotoxic T-lymphocytes (CTLs) must be present in the local circulating HIV-1 strains. Secondly, the vaccine epitopes should match the host population HLA types. We characterized two new HIV-1 isolates from Guinea-Bissau. Also, we have identified 15 subdominant CD8 epitopes representing common HLA super-types theoretically covering most HLA alleles in any population. Herein we demonstrate that the selected vaccine epitopes are well conserved and simultaneously present in sequences from West Africa and Denmark. Use of the selected epitopes will likely ensure ≥10 immune targets in the majority of candidates for experimental therapeutic vaccination in both geographic regions. Our results warrant testing of the selected vaccine epitopes in both geographic locations.
为了使基于 CD8 表位的疫苗适用于不同的地理位置，针对细胞毒性 T 淋巴细胞 (CTL) 的靶向表位必须存在于当地循环的 HIV-1 株中。其次，疫苗表位应与宿主人群 HLA 类型相匹配。我们对来自几内亚比绍的两个新的 HIV-1 分离株进行了特征描述。此外，我们还鉴定了 15 个亚优势 CD8 表位，代表常见的 HLA 超型，理论上覆盖了任何人群中的大多数 HLA 等位基因。在此，我们证明所选疫苗表位具有良好的保守性，同时存在于来自西非和丹麦的序列中。使用所选表位可能会确保在两个地理区域的大多数实验性治疗性疫苗接种候选者中至少有 10 个免疫目标。我们的研究结果证明，在这两个地理区域都需要对所选疫苗表位进行测试。
