Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK.
J Cell Sci. 2011 Aug 1;124(Pt 15):2622-30. doi: 10.1242/jcs.084798. Epub 2011 Jul 12.
A significant number of ciliary disease genes have been found to encode proteins that localise to the basal body. By contrast, a large number of basal-body-associated proteins remain to be characterised. Here, we report the identification of a new basal body protein that is required for ciliogenesis in Drosophila. Dilatory (DILA) is a predicted coiled-coil protein homologous to vertebrate AZI1 (also known as CEP131). Mutations in dila specifically exhibit defects in ciliated cells (sensory neurons and sperm). Several features of the neuronal phenotype suggest a defect in intraflagellar transport. In sensory neuron cilia, DILA protein localises to the ciliary base, including the basal body and putative transition zone, and it interacts genetically with the ciliary coiled-coil protein, Uncoordinated. These data implicate DILA in regulating intraflagellar transport at the base of sensory cilia.
大量的纤毛疾病基因已被发现编码定位于基体的蛋白质。相比之下，大量的基体相关蛋白仍有待表征。在这里，我们报告了一种新的基体蛋白的鉴定，该蛋白是果蝇纤毛发生所必需的。Dilatory（DILA）是一种预测的卷曲螺旋蛋白，与脊椎动物的 AZI1（也称为 CEP131）同源。dila 的突变特异性地表现出纤毛细胞（感觉神经元和精子）的缺陷。神经元表型的几个特征表明内鞭毛运输有缺陷。在感觉神经元纤毛中，DILA 蛋白定位于纤毛基部，包括基体和假定的过渡区，并与纤毛卷曲螺旋蛋白 Uncoordinated 发生遗传相互作用。这些数据表明 DILA 参与调节感觉纤毛基部的内鞭毛运输。
