MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Analyst. 2011 Aug 21;136(16):3343-8. doi: 10.1039/c1an15176b. Epub 2011 Jul 13.
The aggregation-induced emission (AIE) of a 1,2-diphenyl-1,2-di(p-tolyl)ethene (TPE) was explored as a novel fluorescence method for probing the assembling/disassembling of amphiphilic molecules. The fluorescence intensity was able to monitor the formation of micelles and determine the critical micelle concentration (CMC) of surfactants. The temperature-dependent micellization of the pharmaceutically important PEO-PPO-PEO copolymer, Pluronic F127, was further studied by using the TPE fluorescence spectrum intensity. Our results showed good agreement with those reported in the literature by using other methods. The special advantage of the AIE probe method was further explored to determine the assembling/disassembling process of the colored amphiphilic molecule, 1-[4-(3-phenylazophenoxy)butyl]triethylamine bromide (AzoC4), whose CMC value has not previously been described. Since the TPE fluorescence signal mainly comes from the aqueous phase, not from the inside of hydrophobic core, it provides a possible platform to study the CMC of those colored surfactants. Based on the novel fluorescence properties of TPE in the aggregated and dispersed states, one can conclude that the TPE method is a promising method for the determination of the CMC and critical micellization temperature (CMT), particularly having a special advantage to determine the assembling/disassembling process of colored amphiphilic molecules.
聚集诱导发光（AIE）的 1,2-二苯基-1,2-二（对甲苯基）乙烯（TPE）被探索作为一种新的荧光方法来探测两亲分子的组装/解组装。荧光强度能够监测胶束的形成，并确定表面活性剂的临界胶束浓度（CMC）。通过使用 TPE 荧光光谱强度进一步研究了具有重要药用价值的 PEO-PPO-PEO 共聚物 Pluronic F127 的温度依赖性胶束化。我们的结果与使用其他方法在文献中报道的结果吻合良好。通过进一步探索 AIE 探针方法的特殊优势，确定了有色两亲分子 1-[4-(3-苯偶氮苯氧基)丁基]三乙胺溴化物（AzoC4）的组装/解组装过程，其 CMC 值以前没有描述过。由于 TPE 的荧光信号主要来自水相，而不是来自疏水性内核内部，因此它为研究那些有色表面活性剂的 CMC 提供了一个可能的平台。基于 TPE 在聚集态和分散态下的新型荧光性质，可以得出结论，TPE 方法是一种用于确定 CMC 和临界胶束化温度（CMT）的有前途的方法，特别是在确定有色两亲分子的组装/解组装过程方面具有特殊优势。
