利用相差显微镜测量基于天然和荧光染料的单 DNA 分子微芯片动力学。
Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy.
机构信息
Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
出版信息
Chem Commun (Camb). 2011 Aug 28;47(32):9137-9. doi: 10.1039/c1cc12911b. Epub 2011 Jul 13.
PMID:21750823
Abstract
In a free solution of 10.0 mM Gly-Gly (pH 8.2), the flow directions of native-DNA and DNA molecules intercalated with the fluorescent dye YOYO-1 were reversed in the microchip channel. These results clearly showed that in a confined space and under specific environmental conditions, the fluorescent dye modified the original properties and behavior of the native-DNA molecule.
摘要
在 10.0mM Gly-Gly（pH8.2）的游离溶液中，天然 DNA 和与荧光染料 YOYO-1 插入的 DNA 分子在微通道中的流动方向发生反转。这些结果清楚地表明，在受限空间和特定环境条件下，荧光染料改变了天然 DNA 分子的原始性质和行为。
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