Department of Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, PR China.
Oncol Rep. 2011 Nov;26(5):1133-42. doi: 10.3892/or.2011.1387. Epub 2011 Jul 11.
Astrocyte elevated gene-1 (AEG-1), as an HIV-1 or TNF-α-inducible transcript, is correlated with various aspects of tumor malignancy. However, the status of AEG-1 expression and its clinical significance in human gallbladder carcinoma (GBC) remains unknown. In the present study, we investigated AEG-1 expression in two GBC cell lines (GBC-SD and SGC-996) and GBC tissues by immunohistochemical, Western blot and real-time PCR analysis. We found that AEG-1 was highly expressed in GBC samples (63.4%, 26 of 41) compared with normal gallbladder mucosa (p=0.0003) and highly invasive GBC-SD cell lines at both the protein (p=0.0043) and mRNA levels (p=0.0001), and strongly correlated with differentiation degree (p=0.006), Nevin stage (p=0.0344), Ki-67 expression (p=0.0024) and liver infiltration (p=0.0332) in these patients. Multivariate analysis indicated that AEG-1 overexpression was an independent prognostic marker for GBC patients. Moreover, patients with high AEG-1 levels had shorter survival time (p=0.008). Our results suggest that the AEG-1 protein is a valuable marker of GBC progression and could be a potential therapeutic target.
星形细胞上调基因-1（AEG-1）作为 HIV-1 或 TNF-α诱导的转录物，与肿瘤恶性的各个方面相关。然而，AEG-1 在人类胆囊癌（GBC）中的表达状态及其临床意义尚不清楚。在本研究中，我们通过免疫组织化学、Western blot 和实时 PCR 分析研究了 AEG-1 在两种 GBC 细胞系（GBC-SD 和 SGC-996）和 GBC 组织中的表达。我们发现，与正常胆囊黏膜相比，AEG-1 在 GBC 样本中高表达（63.4%，41 例中有 26 例）（p=0.0003），并且在蛋白水平（p=0.0043）和 mRNA 水平（p=0.0001）上均高度表达于侵袭性强的 GBC-SD 细胞系，并且与分化程度（p=0.006）、Nevin 分期（p=0.0344）、Ki-67 表达（p=0.0024）和肝浸润（p=0.0332）密切相关。多因素分析表明，AEG-1 过表达是 GBC 患者的独立预后标志物。此外，AEG-1 水平高的患者生存时间更短（p=0.008）。我们的结果表明，AEG-1 蛋白是 GBC 进展的有价值标志物，可能是潜在的治疗靶点。
