Vega-Vázquez M, Cobas J C, Oliveira de Sousa F F, Martin-Pastor M
Unidade de Resonancia Magnética, Edif. CACTUS, RIAIDT, Campus Vida, University of Santiago de Compostela, A Coruña 15706, Spain.
Magn Reson Chem. 2011 Aug;49(8):464-8. doi: 10.1002/mrc.2786. Epub 2011 Jul 12.
A reverse diffusion filter NMR experiment (Drev) is proposed for the study of small molecules in binding with macromolecules. The filtering efficiency of Drev to eliminate the signals of the macromolecule is shown to be superior to conventional transverse relaxation filters at least for macromolecules containing a significant fraction of flexible residues. The Drev filter was also a useful complement for ligand-based NMR screening in combination with saturation transfer difference experiments.
