University of Minho, Textile Engineering Department, Guimarães, Portugal.
Biotechnol J. 2011 Oct;6(10):1272-9. doi: 10.1002/biot.201100201. Epub 2011 Aug 3.
A laccase-mediated system for denim overdyeing using phenolic compounds was developed. Laccase from ascomycete Myceliophthora thermophila was able to oxidize phenolic compounds such as catechol and catechin and mediate their attachment to denim surfaces. Laccase-generated polymers gave rise to new coloration states from dark brown to green-yellow and replaced dyes in the overdyeing process. Process parameters, such as enzyme dosage, incubation time and presence of mediator, were studied by considering a compromise between the highest overdyeing level and lower energy/products consumption (2 U/mL laccase; 4 h incubation in the absence of mediator). Enzyme-generated polymers were followed by UV/Vis spectrophotometry and their level of attachment to denim surfaces was evaluated by means of spectral values quantification [k/s, Kubelka-Munk relationship (k=absorption coefficient, s=scattering coefficient)]. Overdyeing of denim with phenolics, such as catechol or catechin, was successfully achieved with acceptable levels in terms of durability.
开发了一种使用酚类化合物对牛仔布进行套染的漆酶介导系统。嗜热毁丝霉（Myceliophthora thermophila）来源的漆酶能够氧化儿茶酚和表儿茶素等酚类化合物，并介导它们与牛仔布表面结合。漆酶生成的聚合物赋予了从深棕色到绿黄色的新颜色状态，并在套染过程中取代了染料。通过考虑最高套染水平和较低的能源/产品消耗（2 U/mL 漆酶；在没有介体的情况下孵育 4 小时）之间的折衷，研究了酶剂量、孵育时间和介体存在等工艺参数。通过紫外/可见分光光度法跟踪酶生成的聚合物，并通过定量光谱值评估其在牛仔布表面的附着程度[ k/s，库贝尔卡-芒克关系（k=吸收系数，s=散射系数）]。成功地用儿茶酚或表儿茶素等酚类化合物对牛仔布进行了套染，在耐久性方面达到了可接受的水平。
