诱导子对意大利葡萄品种（Vitis vinifera L. cv Italia）细胞培养物中白藜芦醇和 Viniferin 的产生的影响。

Effects of elicitors on the production of resveratrol and viniferins in cell cultures of Vitis vinifera L. cv Italia.

机构信息

Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

出版信息

J Agric Food Chem. 2011 Sep 14;59(17):9094-101. doi: 10.1021/jf201181n. Epub 2011 Aug 12.

DOI:10.1021/jf201181n

Abstract

Methyl jasmonate, jasmonic acid and chitosan were tested as elicitors on cell suspension cultures obtained from Vitis vinifera cv Italia to investigate their effect on stilbene production. Stilbene accumulation in the callus, grown under nonelicited conditions, was also investigated. Calli and cell suspensions were obtained in a B5 culture medium supplemented with 0.2 mg L(-1) NAA and 1 mg L(-1) KIN. Stilbene determination was achieved by HPLC/DAD/MS. Whereas callus biosynthesized only piceid, cell suspensions elicited with jasmonates produced several stilbenes, mainly viniferins. In suspended cells, methyl jasmonate and jasmonic acid were the most effective in stimulating stilbene biosynthesis, whereas chitosan was less effective; in fact, the amount of stilbenes obtained with this elicitor was not significantly different from that obtained for the control cells. The maximum production of total stilbenes was at day 20 of culture with 0.970 and 1.023 mg g(-1) DW for MeJA and JA, respectively.

摘要

茉莉酸甲酯、茉莉酸和壳聚糖被测试作为诱导子，用于研究它们对葡萄品种意大利（Vitis vinifera cv Italia）悬浮细胞培养物中白藜芦醇生产的影响。还研究了在非诱导条件下生长的愈伤组织中白藜芦醇的积累。在补充了 0.2 mg/L NAA 和 1 mg/L KIN 的 B5 培养基中获得愈伤组织和细胞悬浮液。通过 HPLC/DAD/MS 进行白藜芦醇的测定。愈伤组织仅生物合成白藜芦醇，而用茉莉酸处理的细胞悬浮液则产生了多种白藜芦醇，主要是白藜芦醇。在悬浮细胞中，茉莉酸甲酯和茉莉酸在刺激白藜芦醇生物合成方面最为有效，而壳聚糖的效果则较差；实际上，用这种诱导剂获得的白藜芦醇含量与对照细胞获得的含量没有显著差异。在培养的第 20 天，用 0.970 和 1.023 mg/g DW 的 MeJA 和 JA 分别获得了最大的总白藜芦醇产量。