Miliordos Dimitrios Evangelos, Alatzas Anastasios, Kontoudakis Nikolaos, Kouki Angeliki, Unlubayir Marianne, Gémin Marin-Pierre, Tako Alexandros, Hatzopoulos Polydefkis, Lanoue Arnaud, Kotseridis Yorgos
Laboratory of Oenology and Alcoholic Beverage Drinks, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
Molecular Biology Laboratory, Department of Biotechnology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
Plants (Basel). 2022 Jun 22;11(13):1648. doi: 10.3390/plants11131648.
During the last decade, several studies demonstrated the effect of biostimulants on the transcriptional and metabolic profile of grape berries, suggesting their application as a useful viticultural practice to improve grape and wine quality. Herein, we investigated the impact of two biostimulants-abscisic acid (0.04% / and 0.08% /) and chitosan (0.3% / and 0.6% /)-on the polyphenol metabolism of the Greek grapevine cultivar, Savvatiano, in order to determine the impact of biostimulants' application in the concentration of phenolic compounds. The applications were performed at the veraison stage and the impact on yield, berry quality traits, metabolome and gene expression was examined at three phenological stages (veraison, middle veraison and harvest) during the 2019 and 2020 vintages. Results showed that anthocyanins increased during veraison after treatment with chitosan and abscisic acid. Additionally, stilbenoids were recorded in higher amount following the chitosan and abscisic acid treatments at harvest. Both of the abscisic acid and chitosan applications induced the expression of genes involved in stilbenoids and anthocyanin biosynthesis and resulted in increased accumulation, regardless of the vintage. Alterations in other phenylpropanoid gene expression profiles and phenolic compound concentrations were observed as well. Nevertheless, they were mostly restricted to the first vintage. Therefore, the application of abscisic acid and chitosan on the Greek cultivar Savvatiano showed promising results to induce stilbenoid metabolism and potentially increase grape defense and quality traits.
在过去十年中，多项研究证明了生物刺激剂对葡萄浆果转录和代谢谱的影响，表明其作为一种有用的葡萄栽培实践，可用于提高葡萄和葡萄酒的品质。在此，我们研究了两种生物刺激剂——脱落酸（0.04%和0.08%）和壳聚糖（0.3%和0.6%）——对希腊葡萄品种萨瓦蒂亚诺多酚代谢的影响，以确定生物刺激剂的施用对酚类化合物浓度的影响。在转色期进行处理，并在2019年和2020年葡萄收获季的三个物候阶段（转色期、转色中期和收获期）检测对产量、浆果品质性状、代谢组和基因表达的影响。结果表明，用壳聚糖和脱落酸处理后，转色期花青素含量增加。此外，在收获期，壳聚糖和脱落酸处理后白藜芦醇类化合物的含量更高。脱落酸和壳聚糖的施用均诱导了参与白藜芦醇类化合物和花青素生物合成的基因表达，且无论年份如何，均导致积累增加。还观察到其他苯丙烷类基因表达谱和酚类化合物浓度的变化。然而，它们大多仅限于第一个年份。因此，在希腊品种萨瓦蒂亚诺上施用脱落酸和壳聚糖显示出诱导白藜芦醇类化合物代谢并可能提高葡萄防御能力和品质性状的良好前景。
