Patti A M, Aulicino F A, De Filippis P, Gabrieli R, Volterra L, Panà A
Istituto di Igiene Sanarelli, Università La Sapienza, Roma.
Boll Soc Ital Biol Sper. 1990 Jun;66(6):595-600.
In this research the Enteroviruses presence in sea water was studied. In previous studies presumptive Enteroviruses were revealed in 43% and in 77% of sea water samples analyzed. It was necessary to identify viral particles isolated from marine water because the detection of this kind of virus was performed only on the basis of cytopathic effect appearance on cell cultures. The aim of this research was to verify the suitability of Indirect Immunofluorescence for identification of presumptive Enteroviruses isolated from marine waters. 13 field strains from RC 37 cells (Cercopithecus Kidney cell line) were tested. Pools of Horse immune sera against COXS, POLIO and ECHO, and anti-horse antibodies conjugated with fluorescein were used. The results revealed the presence of Coxsackie virus and Echo virus. There were many problems related with ECHO identification for the presence of cross-reactions with COXS. The IIF method cannot be performed routinely, because it did not show high level of specificity and sensibility, and it is expensive. This methodology can be used only in particular cases and in laboratories with competent virologists and related facilities. In the future, the only suitable methodology for identification of Enteroviruses isolated from field samples is based on the use of RNA and DNA probes.
在本研究中，对海水中肠道病毒的存在情况进行了研究。在之前的研究中，在所分析的海水样本中，分别有43%和77%检测出疑似肠道病毒。有必要对从海水中分离出的病毒颗粒进行鉴定，因为这类病毒的检测仅基于细胞培养中细胞病变效应的出现。本研究的目的是验证间接免疫荧光法对鉴定从海水中分离出的疑似肠道病毒的适用性。对来自RC 37细胞（猕猴肾细胞系）的13株野外菌株进行了检测。使用了针对柯萨奇病毒、脊髓灰质炎病毒和埃可病毒的马免疫血清池，以及与荧光素偶联的抗马抗体。结果显示存在柯萨奇病毒和埃可病毒。由于与柯萨奇病毒存在交叉反应，埃可病毒的鉴定存在许多问题。间接免疫荧光法不能常规使用，因为它没有显示出高特异性和敏感性，而且成本高昂。这种方法仅适用于特定情况以及配备有专业病毒学家和相关设施的实验室。未来，鉴定从野外样本中分离出的肠道病毒的唯一合适方法是基于使用RNA和DNA探针。
