National Key Laboratory of Micro/Nano Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Nanoscale Res Lett. 2011 Jul 13;6(1):450. doi: 10.1186/1556-276X-6-450.
In this study, zinc oxide (ZnO) nanocrystals with different morphologies were synthesized and used as substrates for enzyme immobilization. The effects of morphology of ZnO nanocrystals on enzyme immobilization and their catalytic activities were investigated. The ZnO nanocrystals were prepared through a hydrothermal procedure using tetramethylammonium hydroxide as a mineralizing agent. The control on the morphology of ZnO nanocrystals was achieved by varying the ratio of CH3OH to H2O, which were used as solvents in the hydrothermal reaction system. The surface of as-prepared ZnO nanoparticles was functionalized with amino groups using 3-aminopropyltriethoxysilane and tetraethyl orthosilicate, and the amino groups on the surface were identified and calculated by FT-IR and the Kaiser assay. Horseradish peroxidase was immobilized on as-modified ZnO nanostructures with glutaraldehyde as a crosslinker. The results showed that three-dimensional nanomultipod is more appropriate for the immobilization of enzyme used further in catalytic reaction.
在这项研究中，我们合成了具有不同形态的氧化锌（ZnO）纳米晶体，并将其用作酶固定化的基底。研究了 ZnO 纳米晶体形态对酶固定化及其催化活性的影响。通过使用四甲基氢氧化铵（TMAH）作为矿化剂的水热法制备了 ZnO 纳米晶体。通过改变甲醇（CH3OH）与水（H2O）的比例来控制 ZnO 纳米晶体的形态，该比例用作水热反应体系中的溶剂。使用 3-氨丙基三乙氧基硅烷和正硅酸乙酯对所制备的 ZnO 纳米颗粒的表面进行氨基功能化，并通过傅里叶变换红外光谱（FT-IR）和凯泽试验对表面上的氨基进行鉴定和计算。用过氧化氢酶（HRP）作为交联剂固定在经过修饰的 ZnO 纳米结构上。结果表明，三维纳米多足结构更适合用于进一步的催化反应的酶固定化。
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