MAO Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Res Vet Sci. 2012 Aug;93(1):360-5. doi: 10.1016/j.rvsc.2011.06.013. Epub 2011 Jul 12.
The cytochrome P450 (P450) 3A family is considered to be the most important and abundantly expressed P450 subfamily in mammals. The mRNA expression levels of four P450 3A enzymes in porcine liver and small intestine were investigated using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of P450 3A mRNAs (P450 3A pool) was higher in the liver than that in the small intestine. In the small intestine, the P450 3A mRNAs were gradually decreased from the duodenum to the ileum. P450 3A29 and P450 3A22 were predominantly expressed both in liver and small intestine tissues with larger ratios in the P450 3A pool than the other P450 3A enzymes. These results demonstrate that P450 3A29 and P450 3A22 probably serve as the major P450 3A contributors for both the hepatic and intestinal P450 3A pool. This work provides a deeper comprehension of the contribution of P450 3A enzymes to xenobiotic metabolism in pigs.
细胞色素 P450（P450）3A 家族被认为是哺乳动物中最重要和表达最丰富的 P450 亚家族之一。本研究采用实时逆转录聚合酶链反应（RT-PCR）技术研究了猪肝脏和小肠中 4 种 P450 3A 酶的 mRNA 表达水平。结果表明，P450 3A 酶在肝脏中的表达水平高于小肠。在小肠中，从十二指肠到回肠，P450 3A 酶的 mRNA 逐渐减少。P450 3A29 和 P450 3A22 在肝脏和小肠组织中均大量表达，且在 P450 3A 酶库中的比例大于其他 P450 3A 酶。这些结果表明，P450 3A29 和 P450 3A22 可能是肝脏和肠道 P450 3A 酶库中外源物质代谢的主要 P450 3A 酶。本研究加深了对猪中外源物质代谢中 P450 3A 酶作用的理解。
