二倍体着色性干皮病成纤维细胞的转化与永生化

Transformation and immortalization of diploid xeroderma pigmentosum fibroblasts.

作者信息

Klein B, Pastink A, Odijk H, Westerveld A, van der Eb A J

机构信息

Laboratory for Molecular Carcinogenesis, Syluius Laboratory, Leiden, The Netherlands.

出版信息

Exp Cell Res. 1990 Dec;191(2):256-62. doi: 10.1016/0014-4827(90)90012-y.

DOI:10.1016/0014-4827(90)90012-y

PMID:2175267

Abstract

Diploid xeroderma pigmentosum (XP) skin fibroblast strains from various XP-complementation groups (B, C, G, and H) were transformed with an origin-defective SV40 early region or with the pSV3 gpt plasmid. In the latter case, transfected cells were selected for their ability to express the dominant xgpt gene. Immortalized cell lines were obtained, from XP-complementation groups C (8CA, 3MA, and 20MA; XP3MA and XP20MA were formerly considered to belong to complementation group I), G (2BI and 3BR), and H (2CS). No immortalized cells could be isolated from complementation group B (11BE). The immortalization frequency of wild-type diploid fibroblasts and diploid cultures from XP patients was not significantly increased by cotransfection with the SV40 early region plus several selected viral and cellular oncogenes. In fact, co-transfection with some of the oncogenes caused a marked decrease of the transformation frequency. The observed immortalization occurred at a frequency of approximately 5 x 10(-8).

摘要

来自不同着色性干皮病互补组（B、C、G和H）的二倍体着色性干皮病（XP）皮肤成纤维细胞系用一个缺失原点的SV40早期区域或pSV3 gpt质粒进行转化。在后一种情况下，根据转染细胞表达显性xgpt基因的能力进行筛选。从XP互补组C（8CA、3MA和20MA；XP3MA和XP20MA以前被认为属于互补组I）、G（2BI和3BR）和H（2CS）获得了永生化细胞系。从互补组B（11BE）中未能分离出永生化细胞。野生型二倍体成纤维细胞和XP患者的二倍体细胞培养物与SV40早期区域加上几种选定的病毒和细胞癌基因共转染后，永生化频率没有显著增加。事实上，与某些癌基因共转染导致转化频率显著降低。观察到的永生化发生频率约为5×10^(-8)。