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本文引用的文献

1
Phenotypic analysis of antigen-specific T lymphocytes. Science. 1996. 274: 94-96.抗原特异性T淋巴细胞的表型分析。《科学》。1996年。第274卷：94 - 96页。
J Immunol. 2011 Jul 1;187(1):7-9.
2
High-throughput engineering and analysis of peptide binding to class II MHC.高通量工程技术与 II 类 MHC 肽结合分析。
Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13258-63. doi: 10.1073/pnas.1006344107. Epub 2010 Jul 9.
3
In vivo enhancement of peptide display by MHC class II molecules with small molecule catalysts of peptide exchange.通过肽交换小分子催化剂在体内增强MHC II类分子的肽展示。
J Immunol. 2009 May 15;182(10):6342-52. doi: 10.4049/jimmunol.0803464.
4
Rapid identification of CD4+ T-cell epitopes using yeast displaying pathogen-derived peptide library.利用展示病原体衍生肽库的酵母快速鉴定CD4+ T细胞表位
J Immunol Methods. 2008 Jul 20;336(1):37-44. doi: 10.1016/j.jim.2008.03.008. Epub 2008 Apr 14.
5
Defining the role of the MHC in autoimmunity: a review and pooled analysis.界定主要组织相容性复合体在自身免疫中的作用：一项综述与汇总分析
PLoS Genet. 2008 Apr 25;4(4):e1000024. doi: 10.1371/journal.pgen.1000024.
6
Class II major histocompatibility complex tetramer staining: progress, problems, and prospects.II类主要组织相容性复合体四聚体染色：进展、问题与前景。
Immunology. 2008 Mar;123(3):305-13. doi: 10.1111/j.1365-2567.2007.02801.x.
7
Baculovirus-infected insect cells expressing peptide-MHC complexes elicit protective antitumor immunity.表达肽-主要组织相容性复合体的杆状病毒感染昆虫细胞可引发保护性抗肿瘤免疫。
J Immunol. 2008 Jan 1;180(1):188-97. doi: 10.4049/jimmunol.180.1.188.
8
T cell allorecognition and MHC restriction--A case of Jekyll and Hyde?T细胞同种异体识别与MHC限制——杰基尔与海德的案例？
Mol Immunol. 2008 Feb;45(3):583-98. doi: 10.1016/j.molimm.2006.05.018. Epub 2007 Sep 14.
9
Human TCR-binding affinity is governed by MHC class restriction.人类T细胞受体结合亲和力受主要组织相容性复合体（MHC）类限制的支配。
J Immunol. 2007 May 1;178(9):5727-34. doi: 10.4049/jimmunol.178.9.5727.
10
Display, engineering, and applications of antigen-specific T cell receptors.抗原特异性T细胞受体的展示、工程化及应用
Biomol Eng. 2007 Oct;24(4):361-73. doi: 10.1016/j.bioeng.2007.02.009. Epub 2007 Mar 12.

人 MHC Ⅱ类蛋白的功能细胞表面展示：酵母展示与昆虫细胞展示。

Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display.

机构信息

Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

出版信息

Protein Eng Des Sel. 2011 Sep;24(9):701-9. doi: 10.1093/protein/gzr035. Epub 2011 Jul 13.

DOI:10.1093/protein/gzr035
PMID:21752831
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3160208/
Abstract

Reliable and robust systems for engineering functional major histocompatibility complex class II (MHCII) proteins have proved elusive. Availability of such systems would enable the engineering of peptide-MHCII (pMHCII) complexes for therapeutic and diagnostic applications. In this paper, we have developed a system based on insect cell surface display that allows functional expression of heterodimeric DR2 molecules with or without a covalently bound human myelin basic protein (MBP) peptide, which is amenable to directed evolution of DR2-MBP variants with improved T cell receptor (TCR)-binding affinity. This study represents the first example of functional display of human pMHCII complexes on insect cell surface. In the process of developing this pMHCII engineering system, we have also explored the potential of using yeast surface display for the same application. Our data suggest that yeast display is a useful system for analysis and engineering of peptide binding of MHCII proteins, but not suitable for directed evolution of pMHC complexes that bind with low affinity to self-reactive TCRs.

摘要

可靠且稳健的工程功能性主要组织相容性复合体 II 类 (MHCII) 蛋白的系统一直难以实现。此类系统的可用性将能够为治疗和诊断应用工程化肽-MHCII (pMHCII) 复合物。在本文中，我们开发了一种基于昆虫细胞表面展示的系统，该系统允许功能性表达具有或不具有共价结合的人髓鞘碱性蛋白 (MBP) 肽的异二聚体 DR2 分子，该系统可用于定向进化具有改善的 T 细胞受体 (TCR) 结合亲和力的 DR2-MBP 变体。这项研究代表了在昆虫细胞表面功能性展示人 pMHCII 复合物的首例。在开发这种 pMHCII 工程系统的过程中，我们还探索了使用酵母表面展示进行相同应用的可能性。我们的数据表明，酵母展示是分析和工程 MHCII 蛋白肽结合的有用系统，但不适合结合低亲和力自身反应性 TCR 的 pMHC 复合物的定向进化。