Pourmir Azadeh, Johannes Tyler W
Department of Chemical Engineering, The University of Tulsa, 800 S. Tucker Dr, Tulsa, OK 74104, United States.
Comput Struct Biotechnol J. 2012 Oct 27;2:e201209012. doi: 10.5936/csbj.201209012. eCollection 2012.
Directed evolution has become a well-established tool for improving proteins and biological systems. A critical aspect of directed evolution is the selection of a suitable host organism for achieving functional expression of the target gene. To date, most directed evolution studies have used either Escherichia coli or Saccharomyces cerevisiae as a host; however, other bacterial and yeast species, as well as mammalian and insect cell lines, have also been successfully used. Recent advances in synthetic biology and genomics have opened the possibility of expanding the use of directed evolution to new host organisms such as microalgae. This review focuses on the different host organisms used in directed evolution and highlights some of the recent directed evolution strategies used in these organisms.
定向进化已成为一种完善的用于改进蛋白质和生物系统的工具。定向进化的一个关键方面是选择合适的宿主生物体以实现靶基因的功能性表达。迄今为止，大多数定向进化研究都使用大肠杆菌或酿酒酵母作为宿主；然而，其他细菌和酵母物种，以及哺乳动物和昆虫细胞系也已成功使用。合成生物学和基因组学的最新进展开启了将定向进化的应用扩展到微藻等新宿主生物体的可能性。本综述重点关注定向进化中使用的不同宿主生物体，并突出了这些生物体中最近使用的一些定向进化策略。
