Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
Anatomy and Radiology, Department of Population Health, University of Georgia, Athens, GA, USA.
J Gen Virol. 2011 Nov;92(Pt 11):2586-2589. doi: 10.1099/vir.0.031443-0. Epub 2011 Jul 13.
Glycoprotein J (gJ) of infectious laryngotracheitis virus (ILTV) represents a major viral antigen and is dispensable for replication in cell culture and chickens. We generated gJ deletion mutants derived from the United States Department of Agriculture standard challenge strain (USDA-ch), a GFP-expressing mutant GΔgJ, a gJ deletion mutant void of any foreign DNA insertion (BΔgJ) and a gJ rescue mutant gJR with US5 restored. GΔgJ, BΔgJ and gJR were characterized in cell culture and embryonated eggs. Entry kinetic assays showed that the gJ deletion mutants did not differ in their entry kinetics from gJR. Replication kinetics strongly indicated that gJ plays an important role during egress of the virus. Differences in the abilities of the mutants to replicate in chorioallantoic membranes of chicken embryos and to release infectious virus into the allantoic fluid supported a function of gJ during the egress of ILTV from infected cells.
传染性喉气管炎病毒（ILTV）的糖蛋白 J（gJ）是一种主要的病毒抗原，在细胞培养和鸡中复制时是可有可无的。我们生成了源自美国农业部标准挑战株（USDA-ch）的 gJ 缺失突变体、表达 GFP 的突变体 GΔgJ、不含任何外源 DNA 插入的 gJ 缺失突变体 BΔgJ 和 US5 恢复的 gJ 拯救突变体 gJR。在细胞培养物和鸡胚中对 GΔgJ、BΔgJ 和 gJR 进行了表征。进入动力学测定表明，gJ 缺失突变体在进入动力学方面与 gJR 没有差异。复制动力学强烈表明，gJ 在病毒的出芽过程中起着重要作用。突变体在鸡胚绒毛尿囊膜中的复制能力和向尿囊液中释放感染性病毒的能力的差异支持 gJ 在 ILTV 从受感染细胞中出芽时的作用。
