Department of Pathology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Am J Surg Pathol. 2011 Aug;35(8):1114-22. doi: 10.1097/PAS.0b013e3182233fb6.
Mixed epithelial and stromal tumors are uncommon biphasic tumors of the kidney, with cystic and solid areas composed of a morphologically diverse stroma, including ovarian-like stroma and an epithelial component with considerable heterogeneity. Little is known about the histogenesis and clonal origins of these tumors. A total of 21 mixed epithelial and stromal tumors of the kidney from female patients who underwent radical or partial nephrectomies were examined. The epithelial and stromal components, and also adjacent non-neoplastic renal parenchymal tissues were separately laser microdissected from sections prepared from formalin-fixed and paraffin-embedded tissues for X chromosome inactivation analysis. Nineteen of the 21 tumors were informative. Seven of these informative cases showed random X chromosome inactivation pattern in both epithelial and stromal components of mixed epithelial and stromal tumors of the kidney. Nonrandom inactivation of the X chromosome was found in 12 of 19 informative tumors. The same pattern of nonrandom inactivation of the X chromosome was seen in both epithelial and stromal components in all 12 of the tumors with nonrandom X chromosome inactivation. Our data support the theory that the stroma and epithelium arise from a common cell of origin.
混合型上皮间质肿瘤是一种少见的肾脏两性细胞瘤，由囊性和实性区域组成，包括形态多样的间质，包括卵巢样间质和具有显著异质性的上皮成分。关于这些肿瘤的组织发生和克隆起源知之甚少。共检查了 21 例接受根治性或部分肾切除术的女性患者的混合型上皮间质肿瘤。上皮和间质成分以及相邻的非肿瘤性肾实质组织分别从福尔马林固定和石蜡包埋组织的切片中通过激光微切割进行 X 染色体失活分析。21 个肿瘤中有 19 个是信息性的。在这 7 个信息性病例中，混合型上皮间质肿瘤的上皮和间质成分均显示随机 X 染色体失活模式。在 19 个信息性肿瘤中，有 12 个存在 X 染色体非随机失活。在所有 12 个 X 染色体非随机失活的肿瘤中，上皮和间质成分均观察到相同的 X 染色体非随机失活模式。我们的数据支持这样一种理论，即基质和上皮来自共同的起源细胞。
