Ohta Yuki, Suzuki Noriyuki, Kobayashi Yayoi, Hirano Seishiro
Environmental Nanotoxicology Section, Research Center for Environmental Risk, National Institute for Environmental Studies, Ibaraki, Japan.
Isotopes Environ Health Stud. 2011 Sep;47(3):330-40. doi: 10.1080/10256016.2011.587609. Epub 2011 Jul 15.
Speciation analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP MS) is now commonly used to investigate metabolic and toxicological aspects of some metals and metalloids. We have developed a rapid method for simultaneous identification and quantification of metabolites of selenium (Se) compounds using multiple standards labelled with different isotopes. A mixture of the labelled standards was spiked in a selenised garlic extract and the sample was subjected to speciation analysis by HPLC-ICP MS. The selenised garlic contains γ-glutamyl-methylselenocysteine, methylselenocysteine, and selenomethionine and the concentrations of those Se compounds were 723.8, 414.8, and 310.7 ng Se ml(-1), respectively. The isotopically labelled standards were also applied to the speciation of Se in rat urine. Selenate, methylselenonic acid, selenosugar, and trimethyselenium ions were found to be excreted by the present speciation procedure. Multiple standards labelled with different stable isotopes enable high-throughput identification and quantitative measurements of Se metabolites.
使用高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP MS）进行形态分析，目前常用于研究某些金属和类金属的代谢和毒理学方面。我们开发了一种快速方法，使用不同同位素标记的多种标准品同时鉴定和定量硒（Se）化合物的代谢产物。将标记标准品的混合物加入硒化大蒜提取物中，然后通过HPLC-ICP MS对样品进行形态分析。硒化大蒜含有γ-谷氨酰甲基硒代半胱氨酸、甲基硒代半胱氨酸和硒代蛋氨酸，这些硒化合物的浓度分别为723.8、414.8和310.7 ng Se ml(-1)。同位素标记的标准品也应用于大鼠尿液中硒的形态分析。通过本形态分析方法发现，硒酸盐、甲基硒酸、硒糖和三甲基硒离子会被排出。不同稳定同位素标记的多种标准品能够实现硒代谢产物的高通量鉴定和定量测量。
