运动和 L-精氨酸对发育中大鼠皮质扩散性抑制的差异影响。
Differential effects of physical exercise and L-arginine on cortical spreading depression in developing rats.
机构信息
Departamento de Nutrição, Universidade Federal de Pernambuco, Recife PE, Brazil.
出版信息
Nutr Neurosci. 2011 May;14(3):112-8. doi: 10.1179/1476830511Y.0000000008.
OBJECTIVE
We investigated the effect of early-in-life administration of L-arginine, combined with physical exercise, on cortical spreading depression (CSD) in young and adult rats.
METHODS
L-arginine (300 mg/kg/day, n = 40) or distilled water (vehicle, n = 40) was given to the rats during postnatal days 7-35 by gavage. Physical exercise (treadmill) was carried out during postnatal days 15-35 in half of the animals in each gavage condition described above. The other half (non-exercised) was used for comparison. When the animals reached 35-45 days (young groups) or 90-120 days of age (adult) CSD was recorded on two cortical points during 4 hours and CSD propagation velocity was calculated.
RESULTS
L-arginine-treated + exercised rats had increased body weight, but not brain weight, in adult age compared to L-arginine + non-exercised ones (P < 0.05). In both young and adult animals, L-arginine increased, whereas exercise decreased the CSD propagation velocity. Analysis of variance revealed a significant interaction between gavage treatment and age (P < 0.001), and also between gavage treatment and exercise (P = 0.004), but not between age and exercise. An additional control group of young rats, treated with 300 mg/kg of L-histidine, presented CSD velocities comparable to the corresponding water-treated controls, suggesting that the CSD acceleration seen in the L-arginine group was an L-arginine-specific effect, rather than an effect due to a non-specific amino acid imbalance.
DISCUSSION
L-arginine and exercise affect CSD differentially (L-arginine accelerated, while exercise decelerated CSD), and both effects did interact. Probably, they depend on developmental plasticity changes associated with the treatments.
目的
研究生命早期给予左旋精氨酸(L-arginine)联合运动对幼年和成年大鼠皮质扩散性抑制（CSD）的影响。
方法
通过灌胃给予 L-arginine（300mg/kg/天，n=40）或蒸馏水（载体，n=40），于生后第 7-35 天。在上述两种灌胃条件下的一半动物（运动组）进行生后第 15-35 天的运动（跑步机），另一半（非运动组）用于比较。当动物达到 35-45 天（幼年组）或 90-120 天（成年）时，在两个皮质点记录 4 小时的 CSD，并计算 CSD 传播速度。
结果
与 L-arginine+非运动组相比，L-arginine+运动组成年时体重增加，但脑重无差异（P<0.05）。在幼年和成年动物中，L-arginine 增加，而运动降低 CSD 传播速度。方差分析显示，灌胃处理与年龄（P<0.001）和灌胃处理与运动（P=0.004）之间存在显著交互作用，但年龄与运动之间无交互作用。另一个幼年 L-组氨酸（L-histidine）处理的对照组，CSD 速度与相应的水对照组相当，表明 L-arginine 组中看到的 CSD 加速是 L-arginine 的特异性作用，而不是由于非特异性氨基酸失衡引起的作用。
讨论
L-arginine 和运动对 CSD 的影响不同（L-arginine 加速，而运动减速 CSD），且两种作用相互影响。可能与处理相关的发育可塑性变化有关。
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