Liang Liang, Wang Hong, Peng Xiao-Yan, Zhao Meng
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Zhonghua Yan Ke Za Zhi. 2011 May;47(5):393-7.
To investigate the changes of Th lymphocyte subsets in patients with Behcet disease.
From June 2007 to January 2009, thirty patients (male 16, female 14) with Behcet Disease who got treatment at Beijing Tongren Hospital were collected, whose age range was 21 - 45 years old and the average age was 35 years old. All the patients were diagnosed according to the diagnostic criteria of International Uveitis Study Group, and the other system inflammatory diseases were ruled out. None the patients did receive any anti-inflammatory treatment before getting treatment in the hospital. Thirty health controls (male 15, female 15) were collected in Beijing Tongren Hospital Physical Examination Center. There was on significant difference in age and gender composition between the two groups. Peripheral blood and sera were obtained from 30 patients with Behcet disease and 30 normal persons. The ratio of the subsets of Th cells in peripheral blood was detected by automatic flow cytometer and the IL-17, IL-23, IL-6, IL-10 and TGF-β1 in sera was detected by ELISA. T-test of independent sampler was used to analysis all the scores of the two groups.
Compared to normal controls, the patients with Behcet disease had significant higher percentages of Th1 (25.08% ± 7.23%) and Th17 (36.38% ± 17.47%) (t = -1.43, -2.187; P < 0.05) cells and lower percentages of T regulatory cells (Tregs) (4.11% ± 1.32%) (t = 3.70, P < 0.05), and the Th1/Th2 (13.34 ± 2.66) (t = -1.41, P < 0.05) and Th17/Tregs (9.52 ± 5.82) (t = -2.218, P < 0.05) ratio was higher. No differences in the percentages of Th2 cells between patients with Behcet disease and normal controls were observed. Patients with Behcet disease revealed significant increase in peripheral Th17 related cytokines [IL-17 (13.21 ± 4.20) ng/L, IL-6 (6.37 ± 6.51) ng/L, TGF-β1 (4477.20 ± 2146.60) ng/L, IL-23 (10.86 ± 7.86) ng/L] levels (t = -3.61, -2.33, -2.15 and -3.18; P < 0.05) and obvious decrease in Tregs related cytokines [IL-10 (6.27 ± 5.56) ng/L] (t = 5.95, P < 0.05), compared with normal controls.
There are obvious changes in the number of Th17 and Tregs and their related cytokines during the process of Behcet disease, so it can be inferred that Th17 and Tregs probably have some effects on the onset of Behcet disease.
探讨白塞病患者Th淋巴细胞亚群的变化。
收集2007年6月至2009年1月在北京同仁医院就诊的30例白塞病患者（男16例，女14例），年龄21 - 45岁，平均35岁。所有患者均根据国际葡萄膜炎研究组的诊断标准确诊，排除其他系统炎症性疾病。所有患者入院前均未接受任何抗炎治疗。选取北京同仁医院体检中心30例健康对照者（男15例，女15例）。两组在年龄和性别构成上无显著差异。采集30例白塞病患者和30例正常人的外周血及血清。采用自动流式细胞仪检测外周血Th细胞亚群比例，采用酶联免疫吸附测定法检测血清中IL-17、IL-23、IL-6、IL-10和TGF-β1水平。采用独立样本t检验分析两组各项指标。
与正常对照组相比，白塞病患者Th1细胞（25.08% ± 7.23%）和Th17细胞（36.38% ± 17.47%）百分比显著升高（t = -1.43，-2.187；P < 0.05），调节性T细胞（Tregs）百分比降低（4.11% ± 1.32%）（t = 3.70，P < 0.05），且Th1/Th2比值（13.34 ± 2.66）（t = -1.41，P < 0.05）和Th17/Tregs比值（9.52 ± 5.82）（t = -2.218，P < 0.05）升高。白塞病患者与正常对照组Th2细胞百分比无差异。与正常对照组相比，白塞病患者外周血Th17相关细胞因子[IL-17（13.21 ± 4.20）ng/L，IL-6（6.37 ± 6.51）ng/L，TGF-β1（4477.20 ± 2146.60）ng/L，IL-23（10.86 ± 7.86）ng/L]水平显著升高（t = -3.61，-2.33，-2.15和-3.18；P < 0.05），Tregs相关细胞因子[IL-10（6.27 ± 5.56）ng/L]水平明显降低（t = 5.95，P < 0.05）。
白塞病发病过程中Th17和Tregs数量及其相关细胞因子有明显变化，推测Th17和Tregs可能在白塞病发病中起一定作用。
