Department of Hematology, School of Medicine, Eskişehir Osmangazi University, Eskisehir, 26480, Turkey,
Rheumatol Int. 2013 Dec;33(12):3049-54. doi: 10.1007/s00296-013-2835-8. Epub 2013 Aug 3.
The aim of this study was to investigate the percentage of regulatory T cells and the correlation with clinical activity in Behçet's disease. Forty Behçet's disease (BD) patients, 18 males and 22 females, were included in the study. The patients were diagnosed according to the published BD criteria. Twenty age- and sex-matched volunteer healthy donors were also included. At the time of sampling, clinical activity was assessed for activity signs and symptoms according to the BD Current Activity Form. We considered active those patients with a clinical activity index equal to and/or above 2. Eleven patients had active disease and the remaining were considered as clinically inactive. We have studied the percentages of CD4+CD25+FOXP3+, CD4+CD25+ and CD4+FOXP3+regulatory T cells by flow cytometry and investigated the correlation with disease activity. Percentage of CD4+CD25+FOXP3+Treg in active patients was lower than clinically inactive patients and healthy controls. Percentage of CD4+CD25+Treg was not different between active and inactive patients and healthy controls. Percentage of CD4+FOXP3+Treg was lower than healthy controls in clinically active patients but was not different for inactive group and healthy controls. Patients were active when CD4+CD25+FOXP3+Treg was ≤1.19 %, CD4+CD25+Treg was ≤2.68 %, and CD4+FOXP3+Treg was ≤2.60. CD4+CD25+FOXP3+Treg and CD4+FOXP3+Treg were found negatively correlated with disease activity. Peripheral blood regulatory T cells are decreased in clinically active Behçet's disease patients. The advances in our understanding of the interactions between distinct subsets of Treg and clinical activity might help in modulating BD treatment.
本研究旨在探讨调节性 T 细胞（Treg）的百分比及其与白塞病（BD）临床活动的相关性。共纳入 40 例 BD 患者（男 18 例，女 22 例），根据已发表的 BD 标准进行诊断。同时纳入 20 名年龄和性别匹配的健康志愿者作为对照。在采样时，根据 BD 当前活动表评估了活动体征和症状的临床活动。我们将临床活动指数等于和/或高于 2 的患者视为活动患者。11 例患者患有活动性疾病，其余患者被认为是临床非活动性疾病。我们通过流式细胞术研究了 CD4+CD25+FOXP3+、CD4+CD25+和 CD4+FOXP3+调节性 T 细胞的百分比，并探讨了其与疾病活动的相关性。与临床非活动性患者和健康对照组相比，活动患者的 CD4+CD25+FOXP3+Treg 百分比较低。活性患者和非活性患者与健康对照组之间的 CD4+CD25+Treg 百分比无差异。与健康对照组相比，临床活动患者的 CD4+FOXP3+Treg 百分比较低，但与非活动组和健康对照组无差异。当 CD4+CD25+FOXP3+Treg ≤1.19%、CD4+CD25+Treg ≤2.68%和 CD4+FOXP3+Treg ≤2.60 时，患者处于活动状态。CD4+CD25+FOXP3+Treg 和 CD4+FOXP3+Treg 与疾病活动呈负相关。临床活动期白塞病患者外周血调节性 T 细胞减少。对不同亚群 Treg 与临床活动之间相互作用的深入了解，可能有助于调节 BD 的治疗。
