Zu Ning, Wang Guo-chun
Graduate School, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100032, China.
Zhonghua Yi Xue Za Zhi. 2011 May 10;91(17):1157-60.
To detect the expression levels of interferons IFNα, IFNβ and IFNγ in the peripheral blood and muscle of patients with idiopathic inflammatory myopathies (IIM) so as to analyze their relationships with organ manifestations.
The serum levels of IFNα, IFNβ, IFNγ and anti-Jo-1antibody were measured with ELISA (enzyme-linked immunosorbent assay). And the method of SYBR green dye based real-time quantitative PCR (polymerase chain reaction) was used to compare the expression levels of IFNα, IFNβ, interferon α receptor 1 (IFNαR1), signal transducer and activator of transcription 1 (STAT1) in the IIM patients and normal controls.
The serum levels of IFNα (50.2 ± 21.0) ng/L and IFNβ (10.7 ± 5.4) ng/L in IIM patients were significantly higher than those in normal controls (18.6 ± 3.6) ng/L, (3.9 ± 0.8) ng/L (P < 0.01). And the serum levels of IFNα (55.7 ± 21.9) ng/L and IFNβ (11.3 ± 2.3) ng/L in DM patients were significantly higher than those in PM patients (26.1 ± 8.2) ng/L, (6.2 ± 3.5) ng/L (P < 0.05). The serum levels of IFNα and IFNβ correlated with the presence of skin rash (P < 0.05). And their serum concentrations correlated positively with erythrocyte sedimentation rate (r = 0.442, P = 0.000; r = 0.562, P = 0.000) and C-reactive protein (r = 0.348, P = 0.004; r = 0.432, P = 0.005)respectively. The serum levels of IFNα and IFNβ decreased significantly after treatment in 12 PM/DM (polymyositis/dermatomyositis) patients (P < 0.01). The mRNA expression levels of IFNα, IFNβ, IFNαR1 and STAT1 in PM/DM patients were higher than those in normal controls (P < 0.05).
The expression levels of IFNα and IFNβ in PM/DM patients are significantly higher than those in normal controls. And the increments had correlations with disease activity and severity. STAT1 has been implicated in signal transduction of IFNα and IFNβ in the muscles of PM/DM patients.
检测特发性炎性肌病（IIM）患者外周血及肌肉中干扰素IFNα、IFNβ和IFNγ的表达水平，以分析它们与器官表现的关系。
采用酶联免疫吸附测定法（ELISA）检测IFNα、IFNβ、IFNγ及抗Jo - 1抗体的血清水平。运用基于SYBR绿染料的实时定量聚合酶链反应（PCR）方法，比较IIM患者和正常对照中IFNα、IFNβ、干扰素α受体1（IFNαR1）、信号转导及转录激活因子1（STAT1）的表达水平。
IIM患者血清中IFNα（50.2±21.0）ng/L和IFNβ（10.7±5.4）ng/L显著高于正常对照（18.6±3.6）ng/L、（3.9±0.8）ng/L（P<0.01）。皮肌炎（DM）患者血清中IFNα（55.7±21.9）ng/L和IFNβ（11.3±2.3）ng/L显著高于多发性肌炎（PM）患者（26.1±8.2）ng/L、（6.2±3.5）ng/L（P<0.05）。IFNα和IFNβ的血清水平与皮疹的出现相关（P<0.05）。它们的血清浓度分别与红细胞沉降率（r = 0.442，P = 0.000；r = 0.562，P = 0.000）和C反应蛋白（r = 0.348，P = 0.004；r = 0.432，P = 0.005）呈正相关。12例PM/DM（多发性肌炎/皮肌炎）患者治疗后IFNα和IFNβ的血清水平显著下降（P<0.01）。PM/DM患者中IFNα、IFNβ、IFNαR1和STAT1的mRNA表达水平高于正常对照（P<0.05）。
PM/DM患者中IFNα和IFNβ的表达水平显著高于正常对照。且其升高与疾病活动度和严重程度相关。STAT1参与了PM/DM患者肌肉中IFNα和IFNβ的信号转导。
