Liang Wan-Dong, Yang Ji-Feng, Yan Jun, Jin Jie, Li Ke-Shen, Li Jin-Song, Bi Yun-Tian
School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China.
Zhonghua Yi Xue Za Zhi. 2011 May 17;91(18):1250-3.
To investigate whether the macrophage inflammatory protein 1 alpha (MIP-1α) and apolipoprotein E (ApoE) gene polymorphisms, either alone or in combination, affect the susceptibility to inflammatory bowel disease (IBD).
Genomic DNA of IBD patients with Crohn's disease (CD, n = 41) and with ulcerative colitis (UC, n = 142) and healthy controls (n = 160) was extracted and genotyped for the MIP-1α and ApoE gene polymorphisms by restriction fragment length polymorphism assay.
MIP-1α -906(TA)(6)/(TA)(6) homozygotes had a significantly elevated risk of UC (OR = 1.909, 95%CI = 1.204 - 3.028). The carriers of APOE4ε4 were at a significantly higher risk for UC with OR of 2.379 (95% CI = 1.451 - 3.896). And a combination of these two loci, MIP-1α -906(TA)(6)/(TA)(6)/APOE4ε4 were strongly associated with a higher risk of UC (OR = 3.288; 95%CI = 1.777 - 6.084).
The polymorphisms of MIP-1α -906 (TA)(6)/(TA)(6) and ApoE are probably independent genetic risk factors for UC. And the coexistence of both may exert an additive effect on the UC risks.
研究巨噬细胞炎性蛋白1α（MIP-1α）和载脂蛋白E（ApoE）基因多态性单独或联合是否影响炎性肠病（IBD）易感性。
提取克罗恩病（CD，n = 41）、溃疡性结肠炎（UC，n = 142）患者及健康对照者（n = 160）的基因组DNA，采用限制性片段长度多态性分析法对MIP-1α和ApoE基因多态性进行基因分型。
MIP-1α -906(TA)(6)/(TA)(6)纯合子患UC风险显著升高（OR = 1.909，95%CI = 1.204 - 3.028）。APOE4ε4携带者患UC风险显著更高，OR为2.379（95%CI = 1.451 - 3.896）。这两个位点联合，即MIP-1α -906(TA)(6)/(TA)(6)/APOE4ε4与更高的UC风险密切相关（OR = 3.288；95%CI = 1.777 - 6.084）。
MIP-1α -906(TA)(6)/(TA)(6)和ApoE基因多态性可能是UC独立的遗传危险因素。两者共存可能对UC风险产生累加效应。
