Wang Zhong-yue, Chen Zhao-li, Feng Min, Shi Su-sheng, He Jie, Dai Guang-hai
Department of Oncology, General Hospital of PLA, Beijing 100853, China.
Zhonghua Yi Xue Za Zhi. 2011 May 31;91(20):1397-400.
To investigate the expression and relationship of receptor for activated C kinase 1 (RACK1) and clinical characteristics in esophageal squamous cell carcinoma (ESCC).
Western Blotting was conducted to detect the RACK1 expression in ESCC cell lines. Immunohistochemistry was performed to assay the expression of RACK1 and Ki67 in tumor tissues and adjacent normal epithelium from 113 ESCC patients in tissue microarray. The relationship between the RACK1 level and such clinicopathologic profiles as age, gender, location, smoking, differentiation degree and TNM (tumor, node, metastasis) stage were analyzed.
The expression of RACK1 protein was significantly down-regulated in ESCC tissues as compared with the normal adjacent epithelium (χ(2) = 63.363, P < 0.01). An upregulated expression of RACK1 was observed in 72.5% (29/40) ESCC tissues of patients without a smoking history. And it was significantly higher than that in 46.6% (34/73) of patients with a smoking history (χ(2) = 7.040, P = 0.008). In addition, the rate of up-regulated of RACK1 was significantly higher in stage I and II group (63.8%, 44/69) than that in stage III group (43.2%, 19/44) (χ(2) = 4.616, P = 0.032). Moreover, the ESCC tissues with a higher Ki67 score showed a lower level of RACK1 than that with a lower Ki67 score (χ(2) = 8.261, P = 0.016).
The expression of RACK1 is down-regulated in ESCC tissues and associated with smoking. The expression of RACK1 was associated with smoking, TNM staging and Ki67 score of ESCC.
探讨活化C激酶1受体（RACK1）在食管鳞状细胞癌（ESCC）中的表达、相关性及其与临床特征的关系。
采用蛋白质免疫印迹法检测食管鳞状细胞癌细胞系中RACK1的表达。应用免疫组织化学法检测组织芯片中113例食管鳞状细胞癌患者肿瘤组织及癌旁正常上皮中RACK1和Ki67的表达情况。分析RACK1水平与年龄、性别、肿瘤部位、吸烟、分化程度及TNM（肿瘤、淋巴结、转移）分期等临床病理特征之间的关系。
与癌旁正常上皮相比，食管鳞状细胞癌组织中RACK1蛋白表达明显下调（χ(2)=63.363，P<0.01）。在无吸烟史的患者中，72.5%（29/40）的食管鳞状细胞癌组织RACK1表达上调，显著高于有吸烟史患者中的46.6%（34/73）（χ(2)=7.040，P=0.008）。此外，Ⅰ、Ⅱ期组RACK1上调率（63.8%，44/69）明显高于Ⅲ期组（43.2%，19/44）（χ(2)=4.616，P=0.032）。而且，Ki67评分较高的食管鳞状细胞癌组织中RACK1水平低于Ki67评分较低的组织（χ(2)=8.261，P=0.016）。
食管鳞状细胞癌组织中RACK1表达下调且与吸烟相关。RACK1的表达与食管鳞状细胞癌的吸烟情况、TNM分期及Ki67评分相关。
