Morrisett R A, Mott D D, Lewis D V, Wilson W A, Swartzwelder H S
Department of Medicine (Neurology), Duke University, Durham, NC 27710.
Brain Res Dev Brain Res. 1990 Nov 1;56(2):257-62. doi: 10.1016/0165-3806(90)90090-l.
This study describes the measurement of N-methyl-D-aspartate (NMDA)-mediated excitatory postsynaptic potentials (EPSPs) of the CA1 subregion of transverse hippocampal slices from immature and adult rats. Our methods permit extracellular measurement of NMDA-mediated depolarizations in the presence of magnesium (Mg2+) ions. In comparison to slices from adult rats (75-90 days old), NMDA EPSPs in hippocampus from immature rats (25-35 days old) were of significantly greater amplitude and were significantly less sensitive to magnesium. It is suggested that developmental plasticity may be related to changes in magnesium regulation of the NMDA channel complex.
本研究描述了对未成熟和成年大鼠横向海马切片CA1亚区N-甲基-D-天冬氨酸（NMDA）介导的兴奋性突触后电位（EPSP）的测量。我们的方法允许在镁（Mg2+）离子存在的情况下对NMDA介导的去极化进行细胞外测量。与成年大鼠（75 - 90日龄）的切片相比，未成熟大鼠（25 - 35日龄）海马中的NMDA EPSP具有明显更大的幅度，并且对镁的敏感性明显更低。提示发育可塑性可能与NMDA通道复合物的镁调节变化有关。
