Laboratorio de Etnofarmacología, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico.
J Ethnopharmacol. 2011 Sep 1;137(1):930-3. doi: 10.1016/j.jep.2011.06.028. Epub 2011 Jul 2.
Malmea depressa is traditionally used in the Mayan communities of southeastern Mexico to treat type 2 diabetes. A root bark infusion is being taken throughout the day, between meals.
The aim of this study was to determine whether an ethanolic extract of Malmea depressa would reduce hepatic glucose production by targeting gluconeogenesis. The effects of the plant extract on gluconeogenesis (in vivo) and the activity of GL-6-P (in vitro) were examined.
The plant extract was analyzed by HPLC to confirm its phytochemical composition. The inhibition of gluconeogenesis was tested in vivo by performing a pyruvate tolerance test in n5-STZ after an 18-h fasting period. The extracts effect on glucose-6-phosphatase activity were assayed in vitro with intact rat liver microsomes.
Using HPLC-DAD we confirmed that the phytochemical compositions of the tested extract were similar to those previously reported. We proved that the ethanolic extract of the root bark of Malmea depressa dose-dependently inhibits a glucose peak. Furthermore, the gluconeogenesis inhibition was confirmed in vitro using a pyruvate test.
The results suggest that administration of Malmea depressa can improve glycemic control by blocking hepatic glucose production, especially in the fasting state. These data support its traditional use as an infusion consumed continually throughout the day.
在墨西哥东南部的玛雅社区，Malmea depressa 传统上被用于治疗 2 型糖尿病。人们会在一天中，用餐之间，持续摄入其根皮的煎剂。
本研究旨在确定 Malmea depressa 的乙醇提取物是否可以通过靶向糖异生来降低肝葡萄糖生成。研究了植物提取物对糖异生（体内）和 GL-6-P 活性（体外）的影响。
通过 HPLC 分析植物提取物以确认其植物化学成分。通过在 18 小时禁食期后进行丙酮酸耐量试验，在 n5-STZ 中测试体内糖异生的抑制作用。使用完整大鼠肝微粒体体外测定提取物对葡萄糖-6-磷酸酶活性的影响。
使用 HPLC-DAD，我们证实了测试提取物的植物化学成分与先前报道的相似。我们证明了 Malmea depressa 的根皮乙醇提取物可剂量依赖性地抑制葡萄糖峰值。此外，还通过丙酮酸试验在体外证实了糖异生的抑制作用。
这些结果表明，通过阻断肝葡萄糖生成，给予 Malmea depressa 可以改善血糖控制，特别是在禁食状态下。这些数据支持其作为全天持续摄入的煎剂的传统用途。
