Mindanao State University-Iligan Institute of Technology, Tibanga, Iligan City, Philippines.
Mol Phylogenet Evol. 2011 Nov;61(2):333-50. doi: 10.1016/j.ympev.2011.06.019. Epub 2011 Jul 3.
We investigated phylogeography of Philippine slender toads (genus Ansonia) and used a temporal framework for diversification, statistical tests of alternate topologies, and Bayesian approaches to test previous hypotheses concerning dispersal to, and colonization routes within, the southern Philippine island of Mindanao. Two species of Ansonia previously have been documented, with ranges separated by an east-west split corresponding to the approximate boundaries of Mindanao's paleoisland precursors. We present new mtDNA sequence data (1946 bp from genes encoding ND1, 16S rRNA and tRNALeu) for 105 Ansonia specimens sampled from 20 localities on Mindanao Island. Our data suggest that Philippine Ansonia is composed of at least eight, well-supported population lineages, structured into a minimum of four highly divergent mtDNA clades. One clade corresponds to Ansonia mcgregori, a range-restricted species apparently limited to the distal portion of the Zamboanga Peninsula of western Mindanao. Two morphologically indistinguishable, but genetically divergent, lineages possibly are undescribed cryptic species from western Mindanao. We recognize the five remaining lineages as Ansonia muelleri pending data from morphology or bioacoustics that might diagnose separate species among these lineages. Regardless of their species status, the five allopatric lineages of A. muelleri should be viewed as important genetic units for future genetic conservation planning.
我们研究了菲律宾瘦蟾属（Ansonia）的系统地理学，并利用时间框架、替代拓扑结构的统计检验以及贝叶斯方法来检验先前关于扩散到菲律宾棉兰老岛南部以及该岛内部的殖民路线的假说。此前已经记录了两种 Ansonia 物种，其分布范围被一条东西向的分割线隔开，该分割线大致对应棉兰老岛古岛屿前体的边界。我们为从棉兰老岛 20 个地点采集的 105 个 Ansonia 标本提供了新的 mtDNA 序列数据（来自编码 ND1、16S rRNA 和 tRNALeu 的基因的 1946 个碱基对）。我们的数据表明，菲律宾的 Ansonia 至少由八个得到很好支持的种群谱系组成，这些谱系分为至少四个高度分化的 mtDNA 分支。一个分支与 Ansonia mcgregori 相对应，这是一种范围受限的物种，显然局限于棉兰老岛西部的三宝颜半岛的远端。两个形态上无法区分但遗传上有差异的谱系可能是来自棉兰老岛西部的未描述的隐种。我们将其余的五个谱系识别为 Ansonia muelleri，等待形态学或生物声学方面的数据，这些数据可能会在这些谱系中诊断出不同的物种。无论它们的物种地位如何，这五个地理隔离的 A. muelleri 谱系都应该被视为未来遗传保护规划的重要遗传单位。
