Department of Pathobiology/Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
J Breath Res. 2011 Sep;5(3):037107. doi: 10.1088/1752-7155/5/3/037107. Epub 2011 Jul 15.
For the 2009 influenza A (H1N1) pandemic, vaccination and infection control were the main modes of prevention. A live attenuated H1N1 vaccine mimics natural infection and works by evoking a host immune response, but currently there are no easy methods to measure such a response. To determine if an immune response could be measured in exhaled breath, exhaled nitric oxide (FE(NO)) and other exhaled breath volatiles using selected ion flow tube mass spectrometry (SIFT-MS) were measured before and daily for seven days after administering the H1N1 2009 monovalent live intranasal vaccine (FluMist®, MedImmune LLC) in nine healthy healthcare workers (age 35 ± 7 years; five females). On day 3 after H1N1 FluMist® administration there were increases in FE(NO) (MEAN±SEM: day 0 15 ± 3 ppb, day 3 19 ± 3 ppb; p < 0.001) and breath isoprene (MEAN±SEM: day 0 59 ± 15 ppb, day 3 99 ± 17 ppb; p = 0.02). MS analysis identified the greatest number of changes in exhaled breath on day 3 with 137 product ion masses that changed from baseline. The exhaled breath changes on day 3 after H1N1 vaccination may reflect the underlying host immune response. However, further work to elucidate the sources of the exhaled breath changes is necessary.
针对 2009 年甲型 H1N1 流感大流行，疫苗接种和感染控制是主要的预防措施。减毒活 H1N1 疫苗模拟自然感染，通过引发宿主免疫反应起作用，但目前尚无简便的方法来衡量这种反应。为了确定呼出的一氧化氮（FE（NO））和使用选定的离子流管质谱（SIFT-MS）测量的其他呼出呼吸挥发物是否可以在呼出的呼吸中测量，在九名健康的医疗保健工作者（年龄 35 ± 7 岁；女性五名）中，在接种 2009 年单价活鼻内流感疫苗（FluMist®，MedImmune LLC）之前和之后每天测量 FE（NO）和其他呼出呼吸挥发物。在接种 H1N1 FluMist®后第 3 天，FE（NO）（MEAN±SEM：第 0 天 15 ± 3 ppb，第 3 天 19 ± 3 ppb；p <0.001）和呼气异戊二烯（MEAN±SEM：第 0 天 59 ± 15 ppb，第 3 天 99 ± 17 ppb；p = 0.02）增加。MS 分析在第 3 天鉴定出最多的呼出呼吸变化，有 137 个产物离子质量与基线相比发生变化。接种 H1N1 疫苗后第 3 天呼出的呼吸变化可能反映了潜在的宿主免疫反应。但是，需要进一步的工作来阐明呼出呼吸变化的来源。
