Physikalisch-Technische Bundesanstalt, Bundesallee 100, 38116 Braunschweig, Germany.
Lab Chip. 2011 Sep 7;11(17):2955-60. doi: 10.1039/c1lc20032a. Epub 2011 Jul 15.
We present an on-chip approach for the quantification of biomarkers based on isotope-dilution surface-enhanced Raman scattering (IDSERS). The full procedure was realized on a few square millimetres of a SERS-active substrate, covered with either lithographically engineered gold nanotriangles or silver nanospheres generated by galvanic displacement deposition. The use of certified reference materials combined with the ID principle ensures traceability of the quantitation to SI units. A series of substance spots was deposited onto the SERS active area and measured one by one in fast sequence. The SERS spectra were used to generate and validate a PLS model and also to predict the creatinine concentration of an unknown serum sample.
我们提出了一种基于同位素稀释表面增强拉曼散射（IDSERS）的生物标志物定量的芯片方法。整个过程在几平方毫米的 SERS 活性衬底上实现，该衬底上覆盖有通过光刻工程制造的金纳米三角形或通过电置换沉积生成的银纳米球。使用经过认证的参考材料并结合 ID 原理，可确保定量结果可追溯到 SI 单位。将一系列物质斑点沉积到 SERS 活性区域，并快速顺序逐个进行测量。使用 SERS 光谱生成和验证 PLS 模型，并预测未知血清样品的肌酸酐浓度。
