Imperato A, Honoré T, Jensen L H
Institute of Medical Pharmacology, University La Sapienza, Rome, Italy.
Brain Res. 1990 Oct 22;530(2):223-8. doi: 10.1016/0006-8993(90)91286-p.
Perfusion with quisqualate (5 x 10(-6) M) and kainate (5 x 10(-7) M), selective agonists of glutamate receptors, enhanced the release of dopamine in both caudate and accumbens nuclei of freely-moving rats, measured by the transcerebral microdialysis technique. In contrast, N-methyl-D-aspartate (NMDA) did not affect dopamine release, except at very high concentrations (10(-2) M). The quisqualate-kainate antagonist, FG 9041 (DNQX), antagonized the elevation of dopamine release induced by quisqualate and, furthermore, reduced that of kainate. CPP, a selective NMDA antagonist, did not counteract the quisqualate- or kainate-induced stimulation of dopamine release. The enhancement of dopamine release after quisqualate and kainate was accompanied by behavioural stimulation characterized by grooming, rearing, hypermotility with sniffing and confined sniffing. This behavioural syndrome could be blocked by haloperidol. Conversely, perfusion with NMDA did not activate behaviour even at high concentrations. These results indicate that the dopaminergic system, within the caudate and the accumbens nuclei, is under glutamatergic control through kainate and quisqualate receptors, while the NMDA receptors do not appear to be involved.
使用谷氨酸受体的选择性激动剂喹啉酸（5×10⁻⁶ M）和 kainate（5×10⁻⁷ M）灌注，通过经脑微透析技术测量，可增强自由活动大鼠尾状核和伏隔核中多巴胺的释放。相比之下，N-甲基-D-天冬氨酸（NMDA）除了在非常高的浓度（10⁻² M）下，对多巴胺释放没有影响。喹啉酸-kainate拮抗剂FG 9041（DNQX）可拮抗喹啉酸诱导的多巴胺释放升高，此外，还可降低kainate诱导的升高。选择性NMDA拮抗剂CPP不能抵消喹啉酸或kainate诱导的多巴胺释放刺激。喹啉酸和kainate后多巴胺释放的增强伴随着以梳理、竖毛、伴有嗅探的多动和局限嗅探为特征的行为刺激。这种行为综合征可被氟哌啶醇阻断。相反，即使在高浓度下，用NMDA灌注也不会激活行为。这些结果表明，尾状核和伏隔核内的多巴胺能系统通过kainate和喹啉酸受体受谷氨酸能控制，而NMDA受体似乎不参与其中。
