结直肠癌中侵袭性不同表型的寡核苷酸微阵列表达谱分析

Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer.

作者信息

Thorn Christopher C, Williams Deborah, Freeman Thomas C

机构信息

Department of Academic Surgery, St. James's University Hospital, Leeds, UK.

出版信息

Methods Mol Biol. 2011;755:203-21. doi: 10.1007/978-1-61779-163-5_17.

DOI:10.1007/978-1-61779-163-5_17

PMID:21761306

Abstract

This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.

摘要

本章介绍激光捕获显微切割与寡核苷酸微阵列分析的应用。所描述的方案已成功用于鉴定不同结直肠癌侵袭表型之间的差异转录本表达。内容涵盖组织处理、RNA提取、质量控制、扩增、荧光标记、纯化、杂交以及数据分析要点。