Caruso R L, Juberg D R, Caldwell V, Corcos I A
Department of Environmental and Industrial Health, University of Michigan School of Public Health, Ann Arbor 48109-2029.
Cell Biol Int Rep. 1990 Oct;14(10):905-16. doi: 10.1016/0309-1651(90)91160-6.
Myometrial cells were isolated and cultured from term rat uterus. The myometrial origin of the cultures was verified by antibody staining of cellular desmin and alpha-smooth muscle actin. The presence of functional gap junctions was indicated by transfer of radiolabeled nucleotide and microinjected Lucifer yellow dye. The cultured cells expressed mRNA recognized by a connexin43 gap junction cDNA probe. To our knowledge, this is the first report that isolated myometrial cells form gap junctions in culture.
从足月大鼠子宫中分离并培养子宫肌层细胞。通过细胞结蛋白和α-平滑肌肌动蛋白的抗体染色来验证培养物的子宫肌层来源。通过放射性标记核苷酸的转移和显微注射荧光素黄染料表明存在功能性缝隙连接。培养的细胞表达了可被连接蛋白43缝隙连接cDNA探针识别的mRNA。据我们所知，这是关于分离的子宫肌层细胞在培养中形成缝隙连接的首次报道。
