Kaneko Tomoatsu, Okiji Takashi, Kaneko Reika, Suda Hideaki, Nör Jacques E
Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Niigata, Japan.
Methods Mol Biol. 2011;755:395-403. doi: 10.1007/978-1-61779-163-5_33.
The immunostaining based Laser-capture microdissection (LCM) method called immune-LCM allows us to quantify the mRNA. Immune-LCM has recently been introduced to enhance identification of cells carrying a particular protein from frozen tissue samples. We have recently performed the immune-LCM of formaldehyde-fixated, paraffin-embedded tissues immunostained with a monoclonal antibody Factor VIII. This method could be useful for quantitative gene expression analysis in blood vessels from tumors of patients that have been treated with antiangiogenic drugs, allowing for validation of the effect of drug on the expected targets. Such capability might be exceedingly useful for the evaluation of the bioactivity of new drugs. This method is also useful to compare gene expression patterns in tumor cells versus endothelial cells during tumor progression or tumor angiogenesis.
基于免疫染色的激光捕获显微切割（LCM）方法，即免疫LCM，使我们能够对mRNA进行定量。免疫LCM最近被引入，以增强从冷冻组织样本中识别携带特定蛋白质的细胞的能力。我们最近对用单克隆抗体因子VIII免疫染色的甲醛固定、石蜡包埋组织进行了免疫LCM。该方法可用于抗血管生成药物治疗患者肿瘤血管中的定量基因表达分析，从而验证药物对预期靶点的作用。这种能力对于评估新药的生物活性可能极其有用。该方法也有助于比较肿瘤进展或肿瘤血管生成过程中肿瘤细胞与内皮细胞的基因表达模式。
