Akiyama Kosuke, Miyashita Takenori, Matsubara Ai, Mori Nozomu
Department of Otolaryngology, Faculty of Medicine, Kagawa University, Takamatsu, Kagawa, Japan.
Methods Mol Biol. 2011;755:441-8. doi: 10.1007/978-1-61779-163-5_37.
Laser-capture microdissection (LCM) is an excellent tool to selectively obtain target tissue or cells. The endolymphatic sac (ES) is part of the inner ear, and a large part of the ES is located in the temporal bone. The rat ES is conventionally harvested using stereomicroscopy. In this method, contamination is unavoidable because of its size and location; therefore, additional checks, such as in situ hybridization, are necessary to confirm the cellular localization, and quantitative analysis is difficult in the ES. We have shown a selective epithelial tissue method using LCM to obtain RNA without contamination from ES epithelial tissue.
激光捕获显微切割（LCM）是一种选择性获取目标组织或细胞的出色工具。内淋巴囊（ES）是内耳的一部分，且大部分ES位于颞骨内。传统上使用体视显微镜采集大鼠ES。在这种方法中，由于其大小和位置，污染不可避免；因此，需要进行额外检查，如原位杂交，以确认细胞定位，并且在内淋巴囊中进行定量分析很困难。我们展示了一种使用LCM的选择性上皮组织方法，可从ES上皮组织中无污染地获取RNA。
