Purwosunu Yuditiya, Sekizawa Akihiko, Okai Takashi, Tachikawa Tetsuhiko
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Methods Mol Biol. 2011;755:477-89. doi: 10.1007/978-1-61779-163-5_40.
Preeclampsia is still one of the leading causes of maternal and neonatal mortality and morbidity. Despite intensive research, the cause of preeclampsia has not yet been established. However, for a variety of reasons, the numerous aspects of normal and pathological pregnancies, particularly with regard to preeclampsia, remain difficult to study and are, therefore, poorly understood. The development of a laser-based microdissection system has provided rapid morphologically and phenotypically distinct types of cells in the placenta for molecular analysis. Alterations in gene expression in the cytotrophoblast and syncytiotrophoblast or other specific placental cell types from patients who later develop preeclampsia have been reported. Laser microdissection is an attractive method to study each specific placental cell type to characterize the development of preeclampsia or to study the pathophysiology of preeclampsia. This method may contribute to a better understanding of the pathophysiology of preeclampsia.
子痫前期仍然是孕产妇和新生儿死亡及发病的主要原因之一。尽管进行了深入研究，但子痫前期的病因尚未明确。然而，由于多种原因，正常和病理妊娠的诸多方面，尤其是与子痫前期相关的方面，仍然难以研究，因此了解甚少。基于激光的显微切割系统的开发，为胎盘内形态和表型快速不同的细胞类型进行分子分析提供了条件。有报道称，后来发生子痫前期的患者的细胞滋养层、合体滋养层或其他特定胎盘细胞类型中的基因表达发生了改变。激光显微切割是一种很有吸引力的方法，可用于研究每种特定的胎盘细胞类型，以表征子痫前期的发展或研究子痫前期的病理生理学。这种方法可能有助于更好地理解子痫前期的病理生理学。
