Chengdu Institute of Biology, Chinese Academy of Sciences, PO Box 416, Chengdu 610041, China.
J Proteome Res. 2011 Sep 2;10(9):3944-58. doi: 10.1021/pr200535r. Epub 2011 Aug 2.
Male and female poplar ( Populus cathayana Rehd.) cuttings respond differently to salinity stress. To understand these differences better, comparative morphological, physiological, and proteomics analyses were performed. Treatments with different concentrations of NaCl applied to male and female poplar cuttings for 4 weeks showed that females reacted more negatively at the morphological and physiological levels than did males, visible as shriveled leaves, decreased growth, lowered photosynthetic capacities, and greater Na(+) accumulation. The proteome analysis identified 73 proteins from 82 sexually related salt-responsive spots. They were involved in photosynthesis, protein folding and assembly, synthesis and degradation, carbon, energy and steroid metabolism, plant stress and defense, redox homeostasis, signal transduction, and so forth. The sex-related changes of these proteins were consistent with the different morphological and physiological responses in males and females. In conclusion, the higher salt resistance of male P. cathayana cuttings is related to higher expression and lower degradation of proteins in the photosynthetic apparatus, more effective metabolic mechanism and protective system, and greater capacity of hydrogen peroxide scavenging. This research allows us to further understand the possible different management strategies of cellular activities in male and female Populus when confronted by salt stress.
雄性和雌性白杨（Populus cathayana Rehd.）插条对盐胁迫的反应不同。为了更好地理解这些差异，进行了比较形态学、生理学和蛋白质组学分析。将不同浓度的 NaCl 处理应用于雄性和雌性白杨插条 4 周后发现，雌性在形态和生理水平上的反应比雄性更消极，表现为叶片枯萎、生长减少、光合能力降低和 Na+积累增加。蛋白质组分析从 82 个与性别相关的盐响应斑点中鉴定出 73 种蛋白质。它们参与光合作用、蛋白质折叠和组装、合成和降解、碳、能量和类固醇代谢、植物应激和防御、氧化还原稳态、信号转导等。这些蛋白质的性别相关变化与雄性和雌性之间不同的形态和生理反应一致。总之，雄性 P. cathayana 插条的耐盐性较高与光合作用装置中蛋白质表达水平较高和降解水平较低、更有效的代谢机制和保护系统以及过氧化氢清除能力较大有关。这项研究使我们能够进一步了解在盐胁迫下雄性和雌性杨属细胞活动可能存在不同的管理策略。
