Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, P R China.
Proteomics. 2010 Jul;10(14):2661-77. doi: 10.1002/pmic.200900650.
Drought is a major abiotic stress, limiting the survival and growth of young plants. However, little is known about sex-dependent responses to drought at the proteome level. In this study, we carried out investigations on comparative proteomics, combined with physiological and organelle structure analyses, in males and females of Populus cathayana Rehd. Three-month-old poplar cuttings were treated at 30% of field capacity and at 100% field capacity as a control in a greenhouse for 40 days. Drought greatly inhibited plant growth, damaged the photosynthetic system and destructed the structures of chloroplasts, mitochondria and cellular membranes. However, males suffered less from the adverse effects of drought than did females. Using 2-DE, 563 spots were detected, of which 64 spots displayed significant drought effect and 44 spots displayed a significant sex by drought interaction effect. The results suggest that the different responses to drought stress detected between the sexes have a close relationship to the changes in the expression of sex-dependent proteins, including, e.g. photosynthesis-related proteins, homeostasis-related proteins and stress response proteins. These proteins could contribute to a physiological advantage under drought, giving potential clues for understanding sexual differences in the performance of plants in different environments.
干旱是一种主要的非生物胁迫，限制了幼树的生存和生长。然而，目前对于蛋白质组水平上性别对干旱的依赖响应知之甚少。在这项研究中，我们进行了比较蛋白质组学的研究，结合生理和细胞器结构分析，研究了雄株和雌株的反应。在温室中，将 3 个月大的杨树插条在 30%田间持水量和 100%田间持水量下处理 40 天作为对照。干旱极大地抑制了植物的生长，破坏了光合作用系统，破坏了叶绿体、线粒体和细胞膜的结构。然而，雄性比雌性受干旱不利影响小。通过 2-DE，检测到 563 个斑点，其中 64 个斑点显示出显著的干旱效应，44 个斑点显示出显著的性别与干旱相互作用效应。结果表明，雌雄之间对干旱胁迫的不同响应与性别依赖蛋白表达的变化密切相关，包括光合作用相关蛋白、稳态相关蛋白和应激响应蛋白等。这些蛋白质可能有助于在干旱条件下获得生理优势，为理解植物在不同环境中的性能的性别差异提供了潜在线索。
