Misago Noriyuki, Toda Shuji, Narisawa Yutaka
Division of Dermatology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan.
J Cutan Pathol. 2011 Aug;38(8):609-15. doi: 10.1111/j.1600-0560.2011.01749.x.
There has recently been controversy regarding whether clone My10 is superior to clone QBEND-10 for labeling cells of tricholemmal lineage. Moreover, there have been no previous reports on the CD34 expression in human vellus hair follicles.
We performed a comprehensive study of the CD34 expression in human terminal and vellus hair follicles and in 10 tricholemmomas using both the QBEND-10 and the My10 clones. We also performed two different procedures of immunostaining, which included the using of the standard avidin-biotin-peroxidase (ABC) complex system and the Envision system.
The most sensitive marker of CD34 for normal human hair follicles and tricholemmomas is QBEND-10 using the ABC system. The degree and strength of the CD34 positive staining mainly depended on the method being used (whether it was the ABC system or the Envision system) rather than the clone. CD34 staining was rarely (20-30%) seen in the anagen and catagen vellus hair follicles, and could only be seen by the QBEND-10 clone using the ABC system. CD34 expression in the tricholemmomas represented either a diffuse or peripheral pattern.
CD34 may not be a tricholemmal lineage-specific antigen, but may be related to certain functions of the cells.
最近关于克隆体My10在标记外毛根鞘谱系细胞方面是否优于克隆体QBEND-10存在争议。此外，此前尚无关于人毳毛毛囊中CD34表达的报道。
我们使用QBEND-10和My10克隆体，对人终毛毛囊、毳毛毛囊以及10例外毛根鞘瘤中的CD34表达进行了全面研究。我们还进行了两种不同的免疫染色程序，其中包括使用标准抗生物素蛋白-生物素-过氧化物酶（ABC）复合物系统和Envision系统。
对于正常人毛囊和外毛根鞘瘤，使用ABC系统时，CD34最敏感的标志物是QBEND-10。CD34阳性染色的程度和强度主要取决于所使用的方法（是ABC系统还是Envision系统），而非克隆体。在生长期和退行期毳毛毛囊中很少见到CD34染色（20%-30%），且仅在使用ABC系统的QBEND-10克隆体中可见。外毛根鞘瘤中的CD34表达呈弥漫性或周边性模式。
CD34可能不是外毛根鞘谱系特异性抗原，但可能与细胞的某些功能有关。
