Watanabe K, Yoshida M, Jaffe E A
Second Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1990 Sep;28(9):1214-9.
The activity of angiotensin converting enzyme (ACE) released into the culture medium of human umbilical cord vein endothelial cells in culture (HUVEC) for 24-hour incubation with or without various serotypes of lipopolysaccharide (LPS) was unchanged. ACE activity, however, in cell lysate or monolayer HUVEC after 24-hour incubation of various serotypes of LPS except LPS from E. coli, 026:B6, showed significant decrease, compared with no LPS treatment. Cell lysate contained much higher ACE activity than monolayer HUVEC after 24-hour incubation with or without LPS. These findings give rise to speculation that ACE or ACE-like substance might be located not only on the luminal surface, but also in cytoplasm of HUVEC, and also that decrease of ACE activity in the serum of septic adult respiratory distress syndrome (ARDS) patients might be due to reduced synthesis by vascular endothelial cells.
在培养的人脐静脉内皮细胞（HUVEC）的培养基中，与各种血清型脂多糖（LPS）一起孵育24小时（无论有无LPS），释放的血管紧张素转换酶（ACE）活性未发生变化。然而，除了来自大肠杆菌026:B6的LPS外，各种血清型LPS孵育24小时后，细胞裂解物或单层HUVEC中的ACE活性与未进行LPS处理相比显著降低。与有无LPS孵育24小时后的单层HUVEC相比，细胞裂解物中的ACE活性要高得多。这些发现引发了一种推测，即ACE或ACE样物质可能不仅位于管腔表面，还存在于人脐静脉内皮细胞的细胞质中，并且败血症成人呼吸窘迫综合征（ARDS）患者血清中ACE活性的降低可能是由于血管内皮细胞合成减少所致。
